College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Food Funct. 2020 Mar 26;11(3):2395-2405. doi: 10.1039/c9fo02994j.
Here, we describe a method combining thermo-acid pretreatment and alginate lyase hydrolysis to prepare a low-molecular-weight polysaccharide from the seaweed Laminaria japonica (SP). The in vitro results showed that SP displayed obvious absorption of oil (2.95 g g-1) and cholesterol (21.87 g g-1 at pH 2.0). In addition, the in vivo assessment of SP-related anti-obesity effects in C57BL/6J mice fed a high-fat diet and treated with SP for 8 weeks revealed that SP significantly reduced weight gain and lipid accumulation in white adipose and liver tissues, improved serum lipid profiles, and ameliorated intestinal damage. Moreover, SP activated the AMP-activated protein kinase pathway in liver tissues, downregulated sterol regulatory element-binding protein and fatty acid synthase, and suppressed lipid synthesis. These findings indicated that SP extracted from L. japonica might represent a potent functional food exhibiting anti-obesity effects.
在这里，我们描述了一种结合热酸预处理和海藻酸钠裂解酶水解的方法，从海藻 Laminaria japonica（SP）中制备低分子量多糖。体外结果表明，SP 对油（在 pH 2.0 时为 2.95 gg-1）和胆固醇（21.87 gg-1）具有明显的吸附作用。此外，在 8 周内用 SP 处理高脂饮食喂养的 C57BL/6J 小鼠的 SP 相关抗肥胖作用的体内评估表明，SP 可显著减少白色脂肪组织和肝脏组织中的体重增加和脂质积累，改善血清脂质谱，并改善肠道损伤。此外，SP 激活了肝脏组织中的 AMP 激活的蛋白激酶途径，下调了固醇调节元件结合蛋白和脂肪酸合酶，并抑制了脂质合成。这些发现表明，从 Laminaria japonica 中提取的 SP 可能代表一种具有抗肥胖作用的有效功能食品。
