CINBIO, Universidade de Vigo, Grupo Inmunología, 36310 Vigo, Spain.
CINBIO, Universidade de Vigo, Grupo Biomasa y Desarrollo Sostenible, 32004 Ourense, Spain.
Mar Drugs. 2023 Dec 27;22(1):16. doi: 10.3390/md22010016.
The extract obtained by pressurized hot water extraction from , to recover the bioactive compound known as fucoidan, was fractionated using membranes of 100, 50, 30, 10, and 5 kDa, obtaining five retentates and the final permeate. These fractions were characterized for phloroglucinol content, protein content, sulfate content, and trolox equivalent antioxidant capacity (TEAC); apart from oligosaccharides, FTIR and molar mass distribution were also evaluated. Retentates of 100 and 50 kDa showed higher values for phloroglucinol, TEAC, and sulfate content. The rheology of the alginate fraction was also evaluated. Regarding the potential antitumoral activity, all fractions were assessed in MCF-7 cells using a metabolic activity assay based on the reduction of a tetrazolium compound, the most efficient being R100 and R50. Based on the results, these fractions were compared with commercial fucoidans at the same concentrations, and similar results were found. In addition, synergistic cytotoxic effects using two drugs commonly used in breast cancer, cis-Platinum (cis-Pt) and 5-fluorouracil (5-FU), were tested in combination with R100 and R50. Promising results were obtained when the retentate and the drugs were mixed, showing an improvement in the cytotoxicity induced by the chemotherapy.
采用加压热水提取法从 中提取生物活性化合物褐藻糖胶，使用 100、50、30、10 和 5 kDa 的膜对提取物进行分级，得到五个保留物和最终的渗透物。对这些级分进行了间苯三酚含量、蛋白质含量、硫酸盐含量和 Trolox 当量抗氧化能力 (TEAC) 的分析；除了寡糖之外，还评估了傅里叶变换红外光谱 (FTIR) 和摩尔质量分布。100 和 50 kDa 的保留物具有较高的间苯三酚、TEAC 和硫酸盐含量。还评估了褐藻酸盐级分的流变性。关于潜在的抗肿瘤活性，所有级分均在 MCF-7 细胞中使用基于四唑化合物还原的代谢活性测定法进行评估，其中 R100 和 R50 最为有效。基于这些结果，将这些级分与相同浓度的商业褐藻糖胶进行了比较，发现结果相似。此外，还测试了 R100 和 R50 与两种常用于乳腺癌的药物顺铂 (cis-Pt) 和 5-氟尿嘧啶 (5-FU) 联合使用的协同细胞毒性作用。当保留物和药物混合时，获得了有希望的结果，显示出化疗诱导的细胞毒性的改善。
