单收缩人诱导多能干细胞衍生心肌细胞的制备：基质胶床垫技术
Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique.
作者信息
Cadar Adrian G, Feaster Tromondae K, Durbin Matthew D, Hong Charles C
机构信息
Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee.
Department of Medicine/Division of Cardiovascular Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee.
出版信息
Curr Protoc Stem Cell Biol. 2017 Aug 14;42:4A.14.1-4A.14.7. doi: 10.1002/cpsc.32.
Abstract
This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications. © 2017 by John Wiley & Sons, Inc.
摘要
本单元介绍已发表的基质胶床垫法。简而言之，我们描述了床垫的制备、人诱导多能干细胞衍生的心肌细胞（hiPSC-CM）在基质胶床垫上的重新铺板以及hiPSC-CM床垫的维护。遵循本方案将产生单个的、有力收缩的hiPSC-CM，可用于下游应用。© 2017约翰威立国际出版公司。
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