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Modeling Dengue Virus-Hepatic Cell Interactions Using Human Pluripotent Stem Cell-Derived Hepatocyte-like Cells.利用人多能干细胞衍生的肝细胞样细胞模拟登革热病毒与肝细胞的相互作用
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Hepatocyte-like cells derived from induced pluripotent stem cells.源自诱导多能干细胞的肝样细胞。
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Human induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis B virus.人诱导多能干细胞源性肝细胞系作为乙型肝炎病毒宿主相互作用的新模式。
Sci Rep. 2016 Jul 8;6:29358. doi: 10.1038/srep29358.
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A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host.一种使用源自人类诱导多能干细胞的类肝细胞作为长期宿主的自然丙型肝炎感染的强大模型。
Virol J. 2016 Apr 5;13:59. doi: 10.1186/s12985-016-0519-1.
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Viral hepatitis: A new HCV cell culture model for the next clinical challenges.病毒性肝炎：应对下一个临床挑战的新型 HCV 细胞培养模型。
Nat Rev Gastroenterol Hepatol. 2015 Nov;12(11):611-3. doi: 10.1038/nrgastro.2015.170. Epub 2015 Oct 6.
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SEC14L2 enables pan-genotype HCV replication in cell culture.SEC14L2可使泛基因型丙型肝炎病毒在细胞培养中复制。
Nature. 2015 Aug 27;524(7566):471-5. doi: 10.1038/nature14899. Epub 2015 Aug 12.
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Advances in experimental systems to study hepatitis C virus in vitro and in vivo.体外和体内研究丙型肝炎病毒的实验系统进展。
Virology. 2015 May;479-480:221-33. doi: 10.1016/j.virol.2015.03.014. Epub 2015 Apr 4.
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Human iPSC-derived hepatocyte-like cells support Plasmodium liver-stage infection in vitro.人诱导多能干细胞来源的肝细胞样细胞在体外支持疟原虫肝期感染。
Stem Cell Reports. 2015 Mar 10;4(3):348-59. doi: 10.1016/j.stemcr.2015.01.002. Epub 2015 Feb 7.
9
In vitro infection of primary human hepatocytes by HCV-positive sera: insights on a highly relevant model.用 HCV 阳性血清对原代人肝细胞进行体外感染：一种高度相关模型的研究进展。
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源自人类诱导多能干细胞的类肝细胞作为临床分离丙型肝炎病毒宿主的研究进展

Development of Hepatocyte-like Cell Derived from Human Induced Pluripotent Stem cell as a Host for Clinically Isolated Hepatitis C Virus.

作者信息

Sa-Ngiamsuntorn Khanit, Hongeng Suradej, Wongkajornsilp Adisak

机构信息

Department of Biochemistry, Faculty of Pharmacy, Mahidol University, Rajathevi, Bangkok, Thailand.

Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Rajathevi, Bangkok, Thailand.

出版信息

Curr Protoc Stem Cell Biol. 2017 Aug 14;42(1):4A.13.1-4A.13.34. doi: 10.1002/cpsc.35.

DOI:10.1002/cpsc.35
PMID:28806853
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7162336/
Abstract

This unit describes protocols to develop hepatocyte-like cells (HLCs) starting from mesenchymal stem cells (MSCs) as a natural host for hepatitis C virus (HCV). These include the preparation of MSCs from bone marrow, the reprogramming of MSCs into induced pluripotent stem cells (iPSCs), and the differentiation of iPSCs into HLCs. This unit also incorporates the characterization of the resulting cells at each stage. Another section entails the preparations of HCV. The sources of HCV are either the clinically isolated HCV (HCVser) and the conventional JFH-1 genotype. The last section is the infection protocol coupled with the measurement of viral titer. © 2017 by John Wiley & Sons, Inc.

摘要

本单元描述了从间充质干细胞（MSC）开始培养肝样细胞（HLC）的方案，MSC作为丙型肝炎病毒（HCV）的天然宿主。这些方案包括从骨髓制备MSC、将MSC重编程为诱导多能干细胞（iPSC）以及将iPSC分化为HLC。本单元还包括对每个阶段所得细胞的表征。另一部分涉及HCV的制备。HCV的来源要么是临床分离的HCV（HCVser），要么是传统的JFH-1基因型。最后一部分是感染方案以及病毒滴度的测定。© 2017约翰威立国际出版公司。