源自人类诱导多能干细胞的类肝细胞作为临床分离丙型肝炎病毒宿主的研究进展
Development of Hepatocyte-like Cell Derived from Human Induced Pluripotent Stem cell as a Host for Clinically Isolated Hepatitis C Virus.
作者信息
Sa-Ngiamsuntorn Khanit, Hongeng Suradej, Wongkajornsilp Adisak
机构信息
Department of Biochemistry, Faculty of Pharmacy, Mahidol University, Rajathevi, Bangkok, Thailand.
Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Rajathevi, Bangkok, Thailand.
出版信息
Curr Protoc Stem Cell Biol. 2017 Aug 14;42(1):4A.13.1-4A.13.34. doi: 10.1002/cpsc.35.
This unit describes protocols to develop hepatocyte-like cells (HLCs) starting from mesenchymal stem cells (MSCs) as a natural host for hepatitis C virus (HCV). These include the preparation of MSCs from bone marrow, the reprogramming of MSCs into induced pluripotent stem cells (iPSCs), and the differentiation of iPSCs into HLCs. This unit also incorporates the characterization of the resulting cells at each stage. Another section entails the preparations of HCV. The sources of HCV are either the clinically isolated HCV (HCVser) and the conventional JFH-1 genotype. The last section is the infection protocol coupled with the measurement of viral titer. © 2017 by John Wiley & Sons, Inc.
本单元描述了从间充质干细胞（MSC）开始培养肝样细胞（HLC）的方案，MSC作为丙型肝炎病毒（HCV）的天然宿主。这些方案包括从骨髓制备MSC、将MSC重编程为诱导多能干细胞（iPSC）以及将iPSC分化为HLC。本单元还包括对每个阶段所得细胞的表征。另一部分涉及HCV的制备。HCV的来源要么是临床分离的HCV（HCVser），要么是传统的JFH-1基因型。最后一部分是感染方案以及病毒滴度的测定。© 2017约翰威立国际出版公司。
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