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基于单核苷酸多态性(SNP)标记对田间成熟玉米植株耐盐性主效数量性状位点(QTL)的定位

Mapping of a major QTL for salt tolerance of mature field-grown maize plants based on SNP markers.

作者信息

Luo Meijie, Zhao Yanxin, Zhang Ruyang, Xing Jinfeng, Duan Minxiao, Li Jingna, Wang Naishun, Wang Wenguang, Zhang Shasha, Chen Zhihui, Zhang Huasheng, Shi Zi, Song Wei, Zhao Jiuran

机构信息

Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing, China.

Institute of Crops Research, Hunan Academy of Agricultural Sciences, Changsha, China.

出版信息

BMC Plant Biol. 2017 Aug 15;17(1):140. doi: 10.1186/s12870-017-1090-7.

Abstract

BACKGROUND

Salt stress significantly restricts plant growth and production. Maize is an important food and economic crop but is also a salt sensitive crop. Identification of the genetic architecture controlling salt tolerance facilitates breeders to select salt tolerant lines. However, the critical quantitative trait loci (QTLs) responsible for the salt tolerance of field-grown maize plants are still unknown.

RESULTS

To map the main genetic factors contributing to salt tolerance in mature maize, a double haploid population (240 individuals) and 1317 single nucleotide polymorphism (SNP) markers were employed to produce a genetic linkage map covering 1462.05 cM. Plant height of mature maize cultivated in the saline field (SPH) and plant height-based salt tolerance index (ratio of plant height between saline and control fields, PHI) were used to evaluate salt tolerance of mature maize plants. A major QTL for SPH was detected on Chromosome 1 with the LOD score of 22.4, which explained 31.2% of the phenotypic variation. In addition, the major QTL conditioning PHI was also mapped at the same position on Chromosome 1, and two candidate genes involving in ion homeostasis were identified within the confidence interval of this QTL.

CONCLUSIONS

The detection of the major QTL in adult maize plant establishes the basis for the map-based cloning of genes associated with salt tolerance and provides a potential target for marker assisted selection in developing maize varieties with salt tolerance.

摘要

背景

盐胁迫显著限制植物生长和产量。玉米是一种重要的粮食和经济作物,但也是一种盐敏感作物。鉴定控制耐盐性的遗传结构有助于育种者选择耐盐品系。然而,负责田间种植玉米植株耐盐性的关键数量性状位点(QTL)仍不清楚。

结果

为了定位成熟玉米中耐盐性的主要遗传因素,利用一个双单倍体群体(240个个体)和1317个单核苷酸多态性(SNP)标记构建了一个覆盖1462.05 cM的遗传连锁图谱。用盐田种植的成熟玉米株高(SPH)和基于株高的耐盐指数(盐田与对照田株高之比,PHI)来评价成熟玉米植株的耐盐性。在第1号染色体上检测到一个控制SPH的主效QTL,其LOD值为22.4,解释了31.2%的表型变异。此外,控制PHI的主效QTL也定位在第1号染色体的同一位置,并且在该QTL的置信区间内鉴定出两个参与离子稳态的候选基因。

结论

在成年玉米植株中检测到主效QTL,为耐盐相关基因的图位克隆奠定了基础,并为培育耐盐玉米品种的分子标记辅助选择提供了潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/701a/5556339/d239cd56febb/12870_2017_1090_Fig1_HTML.jpg

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