Hall Barrientos Ivan J, Paladino Eleonora, Szabó Peter, Brozio Sarah, Hall Peter J, Oseghale Charles I, Passarelli Melissa K, Moug Susan J, Black Richard A, Wilson Clive G, Zelkó Romana, Lamprou Dimitrios A
Biomedical Engineering, University of Strathclyde, Glasgow, United Kingdom; Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, United Kingdom.
Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, United Kingdom; EPSRC Centre for Innovative Manufacturing in Continuous Manufacturing and Crystallisation (CMAC), University of Strathclyde, Technology and Innovation Centre, 99 George Street, G1 1RD Glasgow, United Kingdom; National Physical Laboratory (NPL), Hampton Road, Teddington, Middlesex, TW11 0LW, United Kingdom.
Int J Pharm. 2017 Oct 5;531(1):67-79. doi: 10.1016/j.ijpharm.2017.08.071. Epub 2017 Aug 12.
For the creation of scaffolds in tissue engineering applications, it is essential to control the physical morphology of fibres and to choose compositions which do not disturb normal physiological function. Collagen, the most abundant protein in the human body, is a well-established biopolymer used in electrospinning compositions. It shows high in-vivo stability and is able to maintain a high biomechanical strength over time. In this study, the effects of collagen type I in polylactic acid-drug electrospun scaffolds for tissue engineering applications are examined. The samples produced were subsequently characterised using a range of techniques. Scanning electron microscopy analysis shows that the fibre morphologies varied across PLA-drug and PLA-collagen-drug samples - the addition of collagen caused a decrease in average fibre diameter by nearly half, and produced nanofibres. Atomic force microscopy imaging revealed collagen-banding patterns which show the successful integration of collagen with PLA. Solid-state characterisation suggested a chemical interaction between PLA and drug compounds, irgasan and levofloxacin, and the collagen increased the amorphous regions within the samples. Surface energy analysis of drug powders showed a higher dispersive surface energy of levofloxacin compared with irgasan, and contact angle goniometry showed an increase in hydrophobicity in PLA-collagen-drug samples. The antibacterial studies showed a high efficacy of resistance against the growth of both E. coli and S. Aureus, except with PLA-collagen-LEVO which showed a regrowth of bacteria after 48h. This can be attributed to the low drug release percentage incorporated into the nanofibre during the in vitro release study. However, the studies did show that collagen helped shift both drugs into sustained release behaviour. These ideal modifications to electrospun scaffolds may prove useful in further research regarding the acceptance of human tissue by inhibiting the potential for bacterial infection.
在组织工程应用中制备支架时，控制纤维的物理形态并选择不干扰正常生理功能的成分至关重要。胶原蛋白是人体中最丰富的蛋白质，是一种成熟的生物聚合物，用于静电纺丝组合物中。它具有很高的体内稳定性，并且能够长期保持较高的生物力学强度。在本研究中，考察了I型胶原蛋白在用于组织工程应用的聚乳酸-药物静电纺丝支架中的作用。随后使用一系列技术对制备的样品进行了表征。扫描电子显微镜分析表明，聚乳酸-药物和聚乳酸-胶原蛋白-药物样品的纤维形态各不相同——添加胶原蛋白使平均纤维直径减小了近一半，并产生了纳米纤维。原子力显微镜成像揭示了胶原蛋白条带模式，表明胶原蛋白与聚乳酸成功整合。固态表征表明聚乳酸与药物化合物、氯苯甘醚和左氧氟沙星之间存在化学相互作用，并且胶原蛋白增加了样品中的无定形区域。药物粉末的表面能分析表明，与氯苯甘醚相比，左氧氟沙星具有更高的分散表面能，接触角测量表明聚乳酸-胶原蛋白-药物样品的疏水性增加。抗菌研究表明，除了聚乳酸-胶原蛋白-左氧氟沙星在48小时后显示细菌再生长外，对大肠杆菌和金黄色葡萄球菌的生长均具有高效抗性。这可归因于体外释放研究期间纳米纤维中药物释放百分比低。然而，研究确实表明胶原蛋白有助于使两种药物都转变为缓释行为。这些对静电纺丝支架的理想修饰可能在关于抑制细菌感染可能性以促进人体组织接受的进一步研究中证明是有用的。
