Section of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.
Laboratory of Physics, Department of Biotechnology, Agricultural University of Athens, 11855 Athens, Greece.
Mar Drugs. 2022 Oct 25;20(11):664. doi: 10.3390/md20110664.
In search of alternative and sustainable sources of collagenous materials for biomedical applications, the scales of five Mediterranean fish species-fished in high tonnage in the Mediterranean region since they represent popular choices for the local diet-as well as those of the Atlantic salmon for comparison purposes, were comparatively studied for their acid- and pepsin-soluble collagen content. Fish scales that currently represent a discarded biomass of no value could be efficiently exploited for the production of a high added-value biomaterial. The isolated collagenous materials, which showed the typical electrophoretic patterns of type I collagen, were morphologically and physicochemically characterized. Using scanning electron microscopy the fibrous morphology of the isolated collagens was confirmed, while the hydroxyproline content, in conjunction with infrared spectroscopy and X-ray diffraction studies verified the characteristic for collagen amino acid profile and its secondary structure. The acid- and pepsin-soluble collagens isolated from the fish scales were blended with the bioactive sulfated marine polysaccharide ulvan and polyethylene oxide and electrospun to afford nanofibrous scaffolds that could find applications in the biomedical sector.
为了寻找替代和可持续的胶原蛋白材料用于生物医学应用，研究了五种在地中海地区大量捕捞的地中海鱼类的鳞片-由于它们是当地饮食的热门选择-以及大西洋鲑鱼的鳞片，以比较它们的酸溶性和胃蛋白酶溶性胶原蛋白含量。鱼类鳞片目前是一种没有价值的废弃生物质，但可以有效地用于生产高附加值的生物材料。分离出的胶原蛋白材料表现出 I 型胶原蛋白的典型电泳模式，在形态和物理化学方面进行了表征。使用扫描电子显微镜证实了分离胶原蛋白的纤维形态，而羟脯氨酸含量与红外光谱和 X 射线衍射研究一起验证了胶原蛋白氨基酸组成和二级结构的特征。从鱼鳞中分离出的酸溶性和胃蛋白酶溶性胶原蛋白与具有生物活性的海洋硫酸多糖岩藻聚糖和聚氧化乙烯混合，通过静电纺丝制成纳米纤维支架，可用于生物医学领域。
