Hogarth P M, McKenzie I F, Sutton V R, Curnow K M, Lee B K, Eicher E M
Immunogenetics. 1987;25(1):21-7. doi: 10.1007/BF00768829.
The Ly-6 locus is now regarded as a gene complex consisting of at least five closely linked loci (Ly-6A-Ly-6E) whose polymorphic products are identified by monoclonal antibodies and distinguished by different tissue distributions. Ly-6 has been assigned by other investigators to chromosome (Chr) 9 (linked to Thy-1) or to Chr 2. We report that the Ly-6 gene complex, together with the Xp-14 and Gdc-1 loci, is situated on Chr 15 linked to Gpt-1. These new linkage data are derived from four sources: (1) three separate crosses that failed to demonstrate linkage of Ly-6 to either Thy-1 on Chr 9 or to any of five genes present on Chr 2; (2) the NXSM recombinant inbred strains, which suggested the linkage of Ly-6 and Xp-14 to Gpt-1 on Chr 15; (3) several Gpt-1 and Gdc-1 congenic strains that confirmed the assignment of Ly-6 and Xp-14 to Chr 15; and (4) backcrosses that further confirmed the linkage of Ly-6, Gpt-1, Gdc-1, and Xp-14, the probable gene order being Gpt-1/Ly-6-Xp-14-Gdc-1.
Ly-6位点现被视为一个基因复合体，由至少五个紧密连锁的位点（Ly-6A - Ly-6E）组成，其多态性产物可通过单克隆抗体识别，并因不同的组织分布而有所区分。其他研究者已将Ly-6定位于9号染色体（与Thy-1连锁）或2号染色体。我们报告，Ly-6基因复合体与Xp-14和Gdc-1位点一起，位于与Gpt-1连锁的15号染色体上。这些新的连锁数据来自四个来源：（1）三个独立的杂交实验，未能证明Ly-6与9号染色体上的Thy-1或2号染色体上存在的五个基因中的任何一个有连锁关系；（2）NXSM重组近交系，提示Ly-6和Xp-14与15号染色体上的Gpt-1有连锁关系；（3）几个Gpt-1和Gdc-1同源近交系，证实了Ly-6和Xp-14定位于15号染色体；以及（4）回交实验，进一步证实了Ly-6、Gpt-1、Gdc-1和Xp-14的连锁关系，可能的基因顺序为Gpt-1/Ly-6 - Xp-14 - Gdc-1。
