Department of Chemistry, University of California, Irvine, Irvine, CA, 92697, USA.
Sci Rep. 2017 Aug 14;7(1):8074. doi: 10.1038/s41598-017-08520-x.
The complexity of the immune system creates challenges in exploring its importance and robustness. To date, there have been few techniques developed to manipulate individual components of the immune system in an in vivo environment. Here we show a light-based dendritic cell (DC) activation allowing spatial and temporal control of immune activation in vivo. Additionally, we show time dependent changes in RNA profiles of the draining lymph node, suggesting a change in cell profile following DC migration and indicating that the cells migrating have been activated towards antigen presentation.
免疫系统的复杂性给探索其重要性和稳健性带来了挑战。迄今为止，已经开发出了几种技术来在体内环境中操纵免疫系统的单个组件。在这里，我们展示了一种基于光的树突状细胞（DC）激活方法，可实现体内免疫激活的空间和时间控制。此外，我们还显示了引流淋巴结中 RNA 谱的时间依赖性变化，这表明 DC 迁移后细胞表型发生了变化，并表明迁移的细胞已被激活以进行抗原呈递。
