Bharathi Kosaraju, Sreenath H L
Plant Biotechnology Division, Unit of Central Coffee Research Institute, Coffee Board, Mysore, Karnataka, India.
Pharmacogn Mag. 2017 Jul;13(Suppl 2):S196-S200. doi: 10.4103/pm.pm_518_16. Epub 2017 Jul 11.
is the commonly cultivated coffee species in the world along with . Different pests and pathogens affect the production and quality of the coffee. Jasmonic acid (JA) is a plant hormone which plays an important role in plants growth, development, and defense mechanisms, particularly against insect pests. The key enzymes involved in the production of JA are lipoxygenase, allene oxide synthase, allene oxide cyclase, and 12-oxo-phytodienoic reductase. There is no report on the genes involved in JA pathway in coffee plants.
We made an attempt to identify and analyze the genes coding for these enzymes in .
First, protein sequences of jasmonate pathway genes from model plant were identified in the National Center for Biotechnology Information (NCBI) database. These protein sequences were used to search the web-based database Coffee Genome Hub to identify homologous protein sequences in genome using Basic Local Alignment Search Tool (BLAST).
Homologous protein sequences for key genes were identified in the genome database. Protein sequences of the top matches were in turn used to search in NCBI database using BLAST tool to confirm the identity of the selected proteins and to identify closely related genes in species. The protein sequences from database and the top matches in NCBI were aligned, and phylogenetic trees were constructed using MEGA6 software and identified the genetic distance of the respective genes. The study identified the four key genes of JA pathway in , confirming the conserved nature of the pathway in coffee. The study expected to be useful to further explore the defense mechanisms of coffee plants.
JA is a plant hormone that plays an important role in plant defense against insect pests. Genes coding for the 4 key enzymes involved in the production of JA viz., LOX, AOS, AOC, and OPR are identified in (robusta coffee) by bioinformatic approaches confirming the conserved nature of the pathway in coffee. The findings are useful to understand the defense mechanisms of and coffee breeding in the long run.
JA is a plant hormone that plays an important role in plant defense against insect pests. Genes coding for the 4 key enzymes involved in the production of JA viz., LOX, AOS, AOC and OPR were identified and analyzed in (robusta coffee) by in silico approach. The study has confirmed the conserved nature of JA pathway in coffee; the findings are useful to further explore the defense mechanisms of coffee plants. : ; : ; JA: Jasmonic acid; CGH: Coffee Genome Hub; NCBI: National Centre for Biotechnology Information; BLAST: Basic Local Alignment Search Tool; : ; LOX: Lipoxygenase, AOS: Allene oxide synthase; AOC: Allene oxide cyclase; OPR: 12 oxo phytodienoic reductase.
与[另一品种咖啡]一样，[罗布斯塔咖啡]是世界上常见的栽培咖啡品种。不同的害虫和病原体影响咖啡的产量和质量。茉莉酸（JA）是一种植物激素，在植物生长、发育和防御机制中起着重要作用，特别是对害虫的防御。参与茉莉酸合成的关键酶有脂氧合酶、丙二烯氧化物合酶、丙二烯氧化物环化酶和12-氧代植物二烯酸还原酶。目前尚无关于咖啡植物中茉莉酸途径相关基因的报道。
我们试图鉴定和分析[罗布斯塔咖啡]中编码这些酶的基因。
首先，在国家生物技术信息中心（NCBI）数据库中鉴定模式植物中茉莉酸途径基因的蛋白质序列。这些蛋白质序列用于搜索基于网络的数据库咖啡基因组中心（Coffee Genome Hub），使用基本局部比对搜索工具（BLAST）在[罗布斯塔咖啡]基因组中鉴定同源蛋白质序列。
在[罗布斯塔咖啡]基因组数据库中鉴定出关键基因的同源蛋白质序列。将顶级匹配的蛋白质序列依次用于在NCBI数据库中使用BLAST工具进行搜索，以确认所选蛋白质的身份并鉴定物种中的密切相关基因。对[咖啡基因组中心]数据库中的蛋白质序列和NCBI中的顶级匹配序列进行比对，并使用MEGA6软件构建系统发育树，确定各个基因的遗传距离。该研究鉴定了[罗布斯塔咖啡]中茉莉酸途径的四个关键基因，证实了该途径在咖啡中的保守性质。该研究有望有助于进一步探索咖啡植物的防御机制。
茉莉酸是一种在植物抵御害虫中起重要作用的植物激素。通过生物信息学方法在[罗布斯塔咖啡]中鉴定出编码参与茉莉酸合成的4种关键酶即脂氧合酶（LOX）、丙二烯氧化物合酶（AOS）、丙二烯氧化物环化酶（AOC）和12-氧代植物二烯酸还原酶（OPR）的基因，证实了该途径在咖啡中的保守性质。这些发现从长远来看有助于理解[罗布斯塔咖啡]的防御机制和咖啡育种。
茉莉酸是一种在植物抵御害虫中起重要作用的植物激素。通过计算机模拟方法在[罗布斯塔咖啡]中鉴定并分析了编码参与茉莉酸合成的4种关键酶即LOX、AOS、AOC和OPR的基因。该研究证实了咖啡中茉莉酸途径的保守性质；这些发现有助于进一步探索咖啡植物的防御机制。[罗布斯塔咖啡]；[另一品种咖啡]；JA：茉莉酸；CGH：咖啡基因组中心；NCBI：国家生物技术信息中心；BLAST：基本局部比对搜索工具；[罗布斯塔咖啡]；LOX：脂氧合酶，AOS：丙二烯氧化物合酶；AOC：丙二烯氧化物环化酶；OPR：12-氧代植物二烯酸还原酶
