Yin De-Tao, Lei Mengyuan, Xu Jianhui, Li Hongqiang, Wang Yongfei, Liu Zhen, Ma Runsheng, Yu Kun, Li Xianghua
Department of Thyroid Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Key Discipline Laboratory of Clinical Medicine Henan, Zhengzhou, Henan 450050, P.R. China.
Oncol Lett. 2017 Aug;14(2):1309-1314. doi: 10.3892/ol.2017.6317. Epub 2017 Jun 6.
(PV), a traditional Chinese herb, has been shown to be rich in bioactive chemicals and possess anti-proliferative and pro-apoptotic effects on tumor cells. The effect of PV on human well-differentiated thyroid carcinoma (WDTC), which accounts for the majority of common endocrine malignancies, remains to be elucidated. The present study aimed to investigate the function of PV on WDTC cell lines and apoptosis-associated signaling pathway activity. Additional studies demonstrated that PV may induce apoptosis in WDTC TPC-1 and FTC-133 cell lines, using the Cell Counting Kit-8 assay. Morphological changes of apoptotic cells were observed by Hoechst 33342 and acridine orange/ethidium bromide staining. In addition, ladder pattern of fragmented DNA was observed by DNA gel electrophoresis. It was also observed that PV significantly increased Bcl-2-associated X protein and caspase-3 expression, and downregulated B-cell lymphoma-2 expression in TPC-1 and FTC-133 by reverse transcription-quantitative polymerase chain reaction (P<0.05). Thus, the present results indicated that PV has the potential to be a future WDTC therapeutic agent.
夏枯草，一种传统的中草药，已被证明富含生物活性化学物质，并对肿瘤细胞具有抗增殖和促凋亡作用。夏枯草对占常见内分泌恶性肿瘤大多数的人高分化甲状腺癌（WDTC）的作用仍有待阐明。本研究旨在探讨夏枯草对WDTC细胞系的功能及凋亡相关信号通路活性。其他研究表明，使用细胞计数试剂盒-8检测法，夏枯草可能诱导WDTC的TPC-1和FTC-133细胞系凋亡。通过Hoechst 33342和吖啶橙/溴化乙锭染色观察凋亡细胞的形态变化。此外，通过DNA凝胶电泳观察到DNA片段化的梯形条带。通过逆转录-定量聚合酶链反应还观察到，夏枯草显著增加了TPC-1和FTC-133中Bcl-2相关X蛋白和半胱天冬酶-3的表达，并下调了B细胞淋巴瘤-2的表达（P<0.05）。因此，目前的结果表明，夏枯草有可能成为未来WDTC的治疗药物。
