Zhang Ke-Jie, Zhang Ming-Zhi, Wang Qing-Duan, Liu Wen-Li
Department of Hematology Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhong Yao Cai. 2006 Nov;29(11):1207-10.
To investigate the anti-lymphoma effect of Prunella vulgaris L. in order to offer exprimental data for the treatment of lymphoma with Prunella vulgaris L. in clinic.
Effect of Prunella vulgaris L. injection on inhibition ratio of cell growth of Raji cells and IC50 were tested by MTT assay. The growth curve line of Raji cells was drawn also by MTT assay. The cellular morphology was observed by invert microscope, Giemas staining and MTT assay. The expression of apoptosis related protein bcl-2, bax was measured by immunocytochemistry and the quantitative analysis was made with figure analysis system.
The results of immunocytochemistry showed that after Raji cells were treated by the injection of Prunella vulgaris L. (50 mg/ml) for 48 hours, the expression of bcl-1 was up-regulated, and the expression of bax was down-regulated. The differences between process group and control group were significant (P < 0.01).
Prunella vulgaris L. can suppress the proliferation of Raji cells and may be a new anti-lymphoma drug. Inducing the apoptosis of Raji cells maybe one of anti-lymphoma mechanisms.
探讨夏枯草抗淋巴瘤的作用，为临床应用夏枯草治疗淋巴瘤提供实验依据。
采用MTT法检测夏枯草注射液对Raji细胞生长抑制率及IC50，并绘制细胞生长曲线；通过倒置显微镜、吉姆萨染色及MTT法观察细胞形态；采用免疫细胞化学法检测凋亡相关蛋白bcl-2、bax的表达，并运用图像分析系统进行定量分析。
免疫细胞化学结果显示，Raji细胞经50 mg/ml夏枯草注射液作用48小时后，bcl-1表达上调，bax表达下调，处理组与对照组差异有统计学意义（P < 0.01）。
夏枯草能抑制Raji细胞增殖，可能成为新型抗淋巴瘤药物，诱导Raji细胞凋亡可能是其抗淋巴瘤机制之一。
