Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, Key Laboratory of Cluster Science of Ministry of Education, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China.
J Mater Chem B. 2019 May 7;7(17):2829-2834. doi: 10.1039/c8tb03169j. Epub 2019 Apr 1.
Hydrogen selenide (HSe), a central metabolite of Se supplements, displays critical biological functions in many physiological and pathological processes. To better understand its comprehensive function, especially those exerted in subcellular organelles, the development of specific assays is urgently needed. However, the methodology to detect HSe is poorly developed. Here, we present a concise design strategy to obtain an activatable fluorescent probe (Se-1) for HSe by utilizing an intramolecular photoinduced electron transfer (PET) process to switch the fluorescence. The probe is able to selectively react with HSe without interference from intracellular reactive species, and has been successfully used to image the HSe content in lysosomes. Additionally, with the aid of Se-1, we demonstrated that lysosomal HSe can be generated and can gradually accumulate in HepG2 cells under hypoxic conditions. These applications make Se-1 a potential new candidate for deciphering the biological effects of HSe on lysosomes in biology and pathology.
硫化氢（HSe）是硒补充剂的一种重要代谢物，在许多生理和病理过程中具有关键的生物学功能。为了更好地理解其综合功能，特别是在亚细胞细胞器中发挥的功能，迫切需要开发特定的检测方法。然而，检测 HSe 的方法还不够完善。在这里，我们提出了一种简洁的设计策略，通过利用分子内光诱导电子转移（PET）过程来获得一种对 HSe 具有激活荧光的探针（Se-1）来切换荧光。该探针能够选择性地与 HSe 反应，而不受细胞内活性物质的干扰，并已成功用于成像溶酶体中的 HSe 含量。此外，借助 Se-1，我们证明了在缺氧条件下，溶酶体 HSe 可以在 HepG2 细胞中生成并逐渐积累。这些应用使 Se-1 成为在生物学和病理学中解析 HSe 对溶酶体生物学效应的潜在新候选者。
