中国大学生牙龈炎的龈下微生物群

Subgingival Microbiome of Gingivitis in Chinese Undergraduates.

作者信息

Deng Ke, Ouyang Xiang Ying, Chu Yi, Zhang Qian

出版信息

Chin J Dent Res. 2017;20(3):145-152. doi: 10.3290/j.cjdr.a38769.

DOI:10.3290/j.cjdr.a38769

PMID:28808698

Abstract

OBJECTIVE

To analyse the microbiome composition of health and gingivitis in Chinese undergraduates with high-throughput sequencing.

METHODS

Sequencing of 16S rRNA gene amplicons was performed with the MiSeq system to compare subgingival bacterial communities from 54 subjects with gingivitis and 12 periodontally healthy controls.

RESULTS

A total of 1,967,372 sequences representing 14 phyla, 104 genera, and 96 species were detected. Analysis of similarities (Anosim) test and Principal Component Analysis (PCA) showed significantly different community profiles between the health control and the subjects with gingivitis. Alpha-diversity metrics were significantly higher in the subgingival plaque of the subjects with gingivitis compared with that of the healthy control. Overall, the relative abundance of 35 genera and 46 species were significantly different between the two groups, among them 28 genera and 45 species showed higher relative abundance in the subjects with gingivitis, whereas seven genera and one species showed a higher relative abundance in the healthy control. The genera Porphyromonas, Treponema, and Tannerella showed higher relative abundance in the subjects with gingivitis, while the genera Capnocytophaga showed higher proportions in health controls. Porphyromonas gingivalis, Prevotella intermedia and Porphyromonas endodontalis had higher relative abundance in gingivitis. Among them, Porphyromonas gingivalis was most abundant.

CONCLUSION

Our results revealed significantly different microbial community composition and structures of subgingival plaque between subjects with gingivitis and healthy controls. Subjects with gingivitis showed greater taxonomic diversity compared with periodontally healthy subjects. The proportion of Porphyromonas, especially Porphyromonas gingivalis, may be associated with gingivitis subjects aged between 18 and 21 years old in China. Adults with gingivitis in this age group may have a higher risk of developing periodontitis.

摘要

目的

采用高通量测序技术分析中国大学生健康与牙龈炎状态下的微生物群组成。

方法

使用MiSeq系统对16S rRNA基因扩增子进行测序，以比较54例牙龈炎患者和12例牙周健康对照者的龈下细菌群落。

结果

共检测到1,967,372条序列，代表14个门、104个属和96个种。相似性分析（Anosim）测试和主成分分析（PCA）显示，健康对照组与牙龈炎患者的群落图谱存在显著差异。与健康对照组相比，牙龈炎患者龈下菌斑中的α多样性指标显著更高。总体而言，两组之间35个属和46个种的相对丰度存在显著差异，其中28个属和45个种在牙龈炎患者中相对丰度较高，而7个属和1个种在健康对照组中相对丰度较高。卟啉单胞菌属、密螺旋体属和坦纳菌属在牙龈炎患者中相对丰度较高，而二氧化碳嗜纤维菌属在健康对照组中比例较高。牙龈卟啉单胞菌、中间普氏菌和牙髓卟啉单胞菌在牙龈炎中相对丰度较高。其中，牙龈卟啉单胞菌最为丰富。