Oncology Service, Huelva Hospital Complex, Huelva, C/Ronda Norte, s/n, 21005, Huelva, Spain.
Clinical Analysis Service, Huelva Hospital Complex, Huelva, Spain.
Clin Transl Oncol. 2018 Apr;20(4):467-475. doi: 10.1007/s12094-017-1731-1. Epub 2017 Aug 14.
Breast cancer is the most common neoplasm in women and has the highest associated mortality rate. Rapid detection programmes can provide early diagnosis and increase the chances of survival. There are no specific tumor biomarkers for the early phase of the disease. The primary aim of this study was to search a blood biomarker with levels that exceeded the normal range established in the general population that could be used to screen breast cancer.
METHODS/PATIENTS: Case-control study. Conventional as well as research (NGAL, EGFR and 8-OHdG) tumor biomarkers were analyzed.
A total of 126 women were enrolled (cases: 63 patients with local breast cancer; Controls: 63 healthy women). Significant differences were found in patients with higher levels of the conventional markers, Ca15.3, CEA, Cyfra 21.1 and NSE. However, when commercial cut-off values were used, only Ca 15.13 was significant. In the group of research biomarkers, significantly higher levels of EGFR were found in the control group, and of 8-OHdG in the case group. Using logistic regression analysis and a ROC curve, an equation composed of five markers, Ca 15.3, NSE, NGAL, EGFR and 8-OHdG, which yielded a correct diagnostic probability of breast cancer of 91.8% was obtained.
8-OHdG has been identified as a new potential marker for screening early stage breast cancer. In addition, a model that combines five blood markers that can be used as a diagnostic test in certain groups of patients has been developed. New studies with a larger sample size are needed to verify the results obtained.
乳腺癌是女性最常见的肿瘤，其死亡率也最高。快速检测方案可以提供早期诊断并提高生存机会。目前还没有针对疾病早期阶段的特定肿瘤生物标志物。本研究的主要目的是寻找一种血液生物标志物，其水平超过一般人群中建立的正常范围，可用于筛查乳腺癌。
方法/患者：病例对照研究。分析了常规（以及研究）肿瘤生物标志物（NGAL、EGFR 和 8-OHdG）。
共纳入 126 名女性（病例：63 例局部乳腺癌患者；对照组：63 例健康女性）。在常规标志物（CA15.3、CEA、Cyfra 21.1 和 NSE）水平较高的患者中发现了显著差异。然而，当使用商业截断值时，只有 CA15.13 具有显著意义。在研究生物标志物组中，在对照组中发现 EGFR 水平显著升高，而在病例组中发现 8-OHdG 水平显著升高。使用逻辑回归分析和 ROC 曲线，获得了由 CA15.3、NSE、NGAL、EGFR 和 8-OHdG 五种标志物组成的方程，其正确诊断乳腺癌的概率为 91.8%。
8-OHdG 已被确定为筛查早期乳腺癌的新潜在标志物。此外，已经开发出一种模型，该模型结合了五种血液标志物，可以在某些患者群体中用作诊断测试。需要进行更大样本量的新研究来验证所获得的结果。
