Morin Valérie, Véron Nadège, Marcelle Christophe
Institut NeuroMyoGène, INMG, Faculty of Medicine Laënnec, University Lyon1, Bâtiment B, 7 rue Guillaume Paradin, 69008, Lyon, France.
EMBL Australia, Australian Regenerative Medicine Institute, Monash University, 15 Innovation Walk, Clayton, VIC, 3800, Australia.
Methods Mol Biol. 2017;1650:113-123. doi: 10.1007/978-1-4939-7216-6_7.
Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function.
基因组编辑正在推动生物医学科学领域的一场革命，有望为未来的遗传疾病治疗带来希望。RNA引导的基因组编辑CRISPR/Cas9系统已成功应用于修饰多种生物体的基因组。我们最近表明，该技术可与体内电穿孔相结合，以抑制发育中的鸡胚体细胞中感兴趣基因的功能。我们在此展示了先前描述技术的简化版本，该版本可有效导致基因功能丧失。
