Siddiqi Muhammad Zubair, Choi Gyu-Min, Kim Mi-Sun, Im Wan-Taek
Department of Biotechnology, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do 456-749, Republic of Korea.
Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do 456-749, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2665-2671. doi: 10.1099/ijsem.0.001994. Epub 2017 Aug 15.
A Gram-stain-negative, oxidase- and catalase-positive, strictly aerobic, non-gliding bacterial strain, designated as NPT, was isolated from lake water and subjected to a taxonomic study using a polyphasic approach. Colonies of strain NP5T were light-yellow-coloured, circular with regular margins, and opaque. Cells were rods, 0.2-0.5 µm wide and 1.2-3.0 µm long. Strain NP5T possessed β-glycosidase activity, which was responsible for its ability to convert ginsenosides Rb1, Rc and Rd (three main active components of ginseng) to ginsenoside F2. Phylogenetic study based on the 16S rRNA gene sequence put strain NP5T in a distinct lineage in the family Flavobacteriaceae, sharing less than 96.0 % sequence similarity with members of the closely related genera Chryseobacterium, Bergeyella, Epilithonimonas and 'Candidatus Amoebinatus'. The novel isolate showed the highest sequence similarity with the genus Chryseobacterium. Strain NP5T contained MK-6 as predominant quinone, and iso-C15 : 0, iso-C17 : 0 3-OH, iso-C16 : 0 3-OH, C18 : 0 3-OH, anteiso-C15 : 0 and C16 : 1ω7c and/or C16 : 1ω6c (summed feature 3) as major fatty acids. The DNA G+C content was 44.8 mol%. The main polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The physiological, biochemical and taxonomical characteristics allowed the phenotypic differentiation of strain NP5T from its closest phylogenetic neighbours. On the basis of the evidence of this polyphasic study, isolate NP5T represents a novel genus and species in the family Flavobacteriaceae for which the name Daejeonia ginsenosidivorans gen. nov., sp. nov. is proposed. The type strain is NP5T (=KACC 18626T=LMG 29198T).
从湖水中分离出一株革兰氏染色阴性、氧化酶和过氧化氢酶阳性、严格需氧、不滑动的细菌菌株，命名为NPT，并采用多相分类法对其进行分类学研究。菌株NP5T的菌落呈浅黄色，圆形，边缘规则，不透明。细胞呈杆状，宽0.2 - 0.5 µm，长1.2 - 3.0 µm。菌株NP5T具有β - 糖苷酶活性，这使其能够将人参皂苷Rb1、Rc和Rd（人参的三种主要活性成分）转化为人参皂苷F2。基于16S rRNA基因序列的系统发育研究表明，菌株NP5T在黄杆菌科中处于一个独特的谱系，与密切相关的金黄杆菌属、贝氏菌属、附石单胞菌属和“候选变形虫菌属”成员的序列相似性低于96.0%。该新分离菌株与金黄杆菌属显示出最高的序列相似性。菌株NP5T以MK - 6作为主要醌类，以异C15 : 0、异C17 : 0 3 - OH、异C16 : 0 3 - OH、C18 : 0 3 - OH、anteiso - C15 : 0以及C16 : 1ω7c和/或C16 : 1ω6c（总和特征3）作为主要脂肪酸。DNA G + C含量为44.8 mol%。主要极性脂类为磷脂酰乙醇胺、磷脂酰甘油和二磷脂酰甘油。生理、生化和分类学特征使得菌株NP5T能够与其最密切的系统发育邻居进行表型区分。基于这项多相研究的证据，分离株NP5T代表了黄杆菌科中的一个新属和新种，为此提议将其命名为参苷转化大邱菌（Daejeonia ginsenosidivorans），新属，新种。模式菌株为NP5T（=KACC 18626T =LMG 29198T）。
