Thorat Sanjay, Diaspro Alberto, Scarpellini Alice, Povia Mauro, Salerno Marco
Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, Genova I-16163, Italy.
University of Genova, viale Causa 13, Genova I-16145, Italy.
Materials (Basel). 2013 Jan 14;6(1):206-216. doi: 10.3390/ma6010206.
Three different routes were used to infiltrate the pores of anodic porous alumina templates with silver nanoparticles, selected as an example of a bioactive agent. The three methods present a continuous grading from more physical to more chemical character, starting from filling of the pores with pre-existing particles, moving on to formation of particles in the pores by bare calcination and ending with calcination following specific chemical reactions. The resulting presence of silver inside the pores was assessed by means of energy dispersive X-ray spectroscopy and X-ray diffraction. The number and the size of nanoparticles were evaluated by scanning electron microscopy of functionalized alumina cross-sections, followed by image analysis. It appears that the best functionalization results are obtained with the chemical procedure, based on the prior formation of silver ion complex by means of ammonia, followed by reduction with an excess amount of acetaldehyde. Elution of the silver content from the chemically functionalized alumina into phosphate buffer saline has also been examined, demonstrating a sustained release of silver over time, up to 15 h.
以生物活性剂银纳米颗粒为例，采用三种不同途径将其渗入阳极多孔氧化铝模板的孔隙中。这三种方法呈现出从物理性更强到化学性更强的连续梯度变化，起始于用预先存在的颗粒填充孔隙，接着是通过裸烧在孔隙中形成颗粒，最后是经过特定化学反应后进行煅烧。通过能量色散X射线光谱法和X射线衍射评估孔隙内银的存在情况。通过对功能化氧化铝横截面进行扫描电子显微镜观察，随后进行图像分析，来评估纳米颗粒的数量和尺寸。基于通过氨预先形成银离子络合物，随后用过量乙醛还原的化学过程，似乎能获得最佳的功能化效果。还研究了将化学功能化氧化铝中的银含量洗脱到磷酸盐缓冲盐水中的情况，结果表明银会随着时间持续释放，长达15小时。
