• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

扫描开尔文探针显微镜：在生物材料和生物样品上增加应用面临的挑战与前景

Scanning Kelvin Probe Microscopy: Challenges and Perspectives towards Increased Application on Biomaterials and Biological Samples.

作者信息

Salerno Marco, Dante Silvia

机构信息

Materials Characterization Facility, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy.

Department of Nanoscopy & NIC, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy.

出版信息

Materials (Basel). 2018 Jun 5;11(6):951. doi: 10.3390/ma11060951.

DOI:10.3390/ma11060951
PMID:29874810
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6025522/
Abstract

We report and comment on the possible increase of application of scanning Kelvin probe microscopy (SKPM) for biomaterials, biological substrates, and biological samples. First, the fundamental concepts and the practical limitations of SKPM are presented, pointing out the difficulties in proper probe calibration. Then, the most relevant literature on the use of SKPM on biological substrates and samples is briefly reviewed. We report first about biocompatible surfaces used as substrates for subsequent biological applications, such as cultures of living cells. Then, we briefly review the SKPM measurements made on proteins, DNA, and similar biomolecular systems. Finally, some considerations about the perspectives for the use of SKPM in the field of life sciences are made. This work does not pretend to provide a comprehensive view of this emerging scenario, yet we believe that it is time to put these types of application of SKPM under focus, and to face the related challenges, such as measuring in liquid and quantitative comparison with other techniques for the electrical potential readout.

摘要

我们报告并评论扫描开尔文探针显微镜（SKPM）在生物材料、生物基质和生物样品中应用可能增加的情况。首先，介绍了SKPM的基本概念和实际局限性，指出了正确校准探针的困难。然后，简要回顾了关于在生物基质和样品上使用SKPM的最相关文献。我们首先报告用作后续生物应用（如活细胞培养）底物的生物相容性表面。然后，简要回顾了对蛋白质、DNA和类似生物分子系统进行的SKPM测量。最后，对SKPM在生命科学领域的应用前景进行了一些思考。这项工作并非旨在全面呈现这一新兴情况，但我们认为现在是时候关注SKPM的这类应用，并应对相关挑战，如在液体中测量以及与其他电势读出技术进行定量比较。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/e8cc76306f59/materials-11-00951-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/5c08b0cbad63/materials-11-00951-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/fb8b3309235c/materials-11-00951-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/b870a4109a15/materials-11-00951-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/d2ac556cab37/materials-11-00951-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/380c9c80bc42/materials-11-00951-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/8b2790508fe3/materials-11-00951-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/00b8adc9dbc4/materials-11-00951-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/e8cc76306f59/materials-11-00951-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/5c08b0cbad63/materials-11-00951-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/fb8b3309235c/materials-11-00951-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/b870a4109a15/materials-11-00951-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/d2ac556cab37/materials-11-00951-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/380c9c80bc42/materials-11-00951-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/8b2790508fe3/materials-11-00951-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/00b8adc9dbc4/materials-11-00951-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481a/6025522/e8cc76306f59/materials-11-00951-g008.jpg

相似文献

1
Scanning Kelvin Probe Microscopy: Challenges and Perspectives towards Increased Application on Biomaterials and Biological Samples.扫描开尔文探针显微镜：在生物材料和生物样品上增加应用面临的挑战与前景
Materials (Basel). 2018 Jun 5;11(6):951. doi: 10.3390/ma11060951.
2
Quantitative amplitude-modulation scanning Kelvin probe microscopy via the second eigenmode excitation.通过第二本征模激发的定量幅度调制扫描开尔文探针显微镜。
Ultramicroscopy. 2021 Nov;230:113399. doi: 10.1016/j.ultramic.2021.113399. Epub 2021 Sep 26.
3
Intensity-modulated scanning Kelvin probe microscopy for probing recombination in organic photovoltaics.用于探测有机光伏器件中复合的调制扫描开尔文探针显微镜。
ACS Nano. 2014 Oct 28;8(10):10799-807. doi: 10.1021/nn5045867. Epub 2014 Sep 15.
4
Built-in potential and charge distribution within single heterostructured nanorods measured by scanning Kelvin probe microscopy.通过扫描开尔文探针显微镜测量单个异质结构纳米棒内的内置电势和电荷分布。
Nano Lett. 2013 Mar 13;13(3):1278-84. doi: 10.1021/nl4000147. Epub 2013 Feb 8.
5
Direct imaging of defect formation in strained organic flexible electronics by Scanning Kelvin Probe Microscopy.扫描开尔文探针显微镜直接观察应变有机柔性电子器件中的缺陷形成。
Sci Rep. 2016 Dec 2;6:38203. doi: 10.1038/srep38203.
6
A scanning Kelvin probe study of charge trapping in zone-cast pentacene thin film transistors.区域铸造并五苯薄膜晶体管中电荷俘获的扫描开尔文探针研究。
Nanotechnology. 2009 Jan 14;20(2):025203. doi: 10.1088/0957-4484/20/2/025203. Epub 2008 Dec 9.
7
Real versus measured surface potentials in scanning Kelvin probe microscopy.扫描开尔文探针显微镜中真实表面电位与测量表面电位的对比
ACS Nano. 2008 Apr;2(4):622-6. doi: 10.1021/nn700190t.
8
Functional Scanning Probe Imaging of Nanostructured Solar Energy Materials.功能扫描探针成像在纳米结构太阳能材料中的应用。
Acc Chem Res. 2016 Sep 20;49(9):1769-76. doi: 10.1021/acs.accounts.6b00255. Epub 2016 Aug 30.
9
Applying the Kelvin probe to biological tissues: theoretical and computational analyses.将开尔文探针应用于生物组织：理论与计算分析。
Phys Rev E Stat Nonlin Soft Matter Phys. 2012 Jun;85(6 Pt 1):061901. doi: 10.1103/PhysRevE.85.061901. Epub 2012 Jun 1.
10
Tuning the graphene work function by electric field effect.通过电场效应调节石墨烯的功函数。
Nano Lett. 2009 Oct;9(10):3430-4. doi: 10.1021/nl901572a.

引用本文的文献

1
Electric Field Cycling of Physisorbed Antibodies Reduces Biolayer Polarization Dispersion.物理吸附抗体的电场循环可降低生物层极化色散。
Adv Sci (Weinh). 2025 Jan;12(1):e2412347. doi: 10.1002/advs.202412347. Epub 2024 Nov 8.
2
Mapping elemental solutes at sub-picogram levels during aqueous corrosion of Al alloys using diffusive gradients in thin films (DGT) with LA-ICP-MS.使用带激光烧蚀电感耦合等离子体质谱仪（LA-ICP-MS）的薄膜扩散梯度技术（DGT）对铝合金水腐蚀过程中亚皮克级的元素溶质进行测绘。
Anal Bioanal Chem. 2024 Jun;416(14):3373-3388. doi: 10.1007/s00216-024-05288-8. Epub 2024 Apr 16.
3
Droplet epitaxy of InGaN quantum dots on Si (111) by plasma-assisted molecular beam epitaxy.

本文引用的文献

1
Developmental refinement of synaptic transmission on micropatterned single layer graphene.在微图案化单层石墨烯上发育性改善突触传递。
Acta Biomater. 2018 Jan;65:363-375. doi: 10.1016/j.actbio.2017.11.005. Epub 2017 Nov 6.
2
Charge distribution from SKPM images.扫描开尔文探针显微镜（SKPM）图像的电荷分布
Phys Chem Chem Phys. 2017 Oct 18;19(40):27299-27304. doi: 10.1039/c7cp05401g.
3
Amyloid and membrane complexity: The toxic interplay revealed by AFM.淀粉样蛋白和膜复杂性：原子力显微镜揭示的毒性相互作用。
通过等离子体辅助分子束外延在Si(111)上进行InGaN量子点的液滴外延生长。
Discov Nano. 2023 Apr 7;18(1):60. doi: 10.1186/s11671-023-03844-2.
4
Boosting the Photoelectrochemical Performance of Au/ZnO Nanorods by Co-Occurring Gradient Doping and Surface Plasmon Modification.通过共掺杂梯度和表面等离子体修饰来提高 Au/ZnO 纳米棒的光电化学性能。
Int J Mol Sci. 2022 Dec 27;24(1):443. doi: 10.3390/ijms24010443.
5
Molecular Doping of CVD-Graphene Surfaces by Perfluoroalkyl-Substituted Perylene Diimides Derivatives.全氟烷基取代苝二酰亚胺衍生物对化学气相沉积法制备的石墨烯表面进行分子掺杂
Nanomaterials (Basel). 2022 Nov 28;12(23):4239. doi: 10.3390/nano12234239.
6
Application of Poly-L-Lysine for Tailoring Graphene Oxide Mediated Contact Formation between Lithium Titanium Oxide LTO Surfaces for Batteries.聚-L-赖氨酸在定制氧化石墨烯介导的锂钛氧化物（LTO）电池表面间接触形成中的应用
Polymers (Basel). 2022 May 25;14(11):2150. doi: 10.3390/polym14112150.
7
Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection.具有抗菌活性的 Photolon 纳米多孔光活性材料及用于自由基检测的无标记非接触方法。
Int J Mol Sci. 2021 Dec 28;23(1):279. doi: 10.3390/ijms23010279.
8
Developments and Ongoing Challenges for Analysis of Surface-Bound Proteins.表面结合蛋白分析的发展与挑战
Annu Rev Anal Chem (Palo Alto Calif). 2021 Jul 27;14(1):389-412. doi: 10.1146/annurev-anchem-091520-010206.
9
A study of dopant incorporation in Te-doped GaAsSb nanowires using a combination of XPS/UPS, and C-AFM/SKPM.一项使用XPS/UPS与C-AFM/SKPM相结合的方法对碲掺杂的砷化镓锑纳米线中掺杂剂掺入情况的研究。
Sci Rep. 2021 Apr 15;11(1):8329. doi: 10.1038/s41598-021-87825-4.
10
Bulk chemical composition contrast from attractive forces in AFM force spectroscopy.原子力显微镜力谱中吸引力引起的整体化学成分对比。
Beilstein J Nanotechnol. 2021 Jan 18;12:58-71. doi: 10.3762/bjnano.12.5. eCollection 2021.
Semin Cell Dev Biol. 2018 Jan;73:82-94. doi: 10.1016/j.semcdb.2017.08.046. Epub 2017 Aug 30.
4
Comparative Study of Loading of Anodic Porous Alumina with Silver Nanoparticles Using Different Methods.使用不同方法将银纳米颗粒负载到阳极多孔氧化铝上的比较研究。
Materials (Basel). 2013 Jan 14;6(1):206-216. doi: 10.3390/ma6010206.
5
Fabrication of Gold-Coated Ultra-Thin Anodic Porous Alumina Substrates for Augmented SERS.用于增强表面增强拉曼光谱的镀金超薄阳极多孔氧化铝基底的制备
Materials (Basel). 2016 May 24;9(6):403. doi: 10.3390/ma9060403.
6
Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina.自组装硫醇单分子层以及在薄阳极多孔氧化铝上的支撑脂质膜的表面增强拉曼散射
Beilstein J Nanotechnol. 2017 Jan 9;8:74-81. doi: 10.3762/bjnano.8.8. eCollection 2017.
7
Endogenous electric fields as guiding cue for cell migration.内源性电场作为细胞迁移的引导线索。
Front Physiol. 2015 May 13;6:143. doi: 10.3389/fphys.2015.00143. eCollection 2015.
8
Surface potential measurement of bacteria using Kelvin probe force microscopy.使用开尔文探针力显微镜对细菌进行表面电位测量。
J Vis Exp. 2014 Nov 28(93):e52327. doi: 10.3791/52327.
9
Nanoporous anodic alumina platforms: engineered surface chemistry and structure for optical sensing applications.纳米多孔阳极氧化铝平台：用于光学传感应用的工程化表面化学与结构
Sensors (Basel). 2014 Jul 7;14(7):11878-918. doi: 10.3390/s140711878.
10
Visualizing and quantifying charge distributions correlated to threshold voltage shifts in lateral organic transistors.可视化和量化与横向有机晶体管阈值电压漂移相关的电荷分布。
ACS Nano. 2014 Mar 25;8(3):2714-24. doi: 10.1021/nn4064067. Epub 2014 Feb 24.