在黄酮类化合物诱导前列腺癌细胞产生细胞毒性的过程中，前列腺干细胞抗原的表达下调。
Expression of prostate stem cell antigen is downregulated during flavonoid-induced cytotoxicity in prostate cancer cells.
作者信息
Zhang Qiang, Cheng Guangdong, Qiu Hongbin, Wang Yuexin, Wang Jingtao, Xu Hui, Zhang Tao, Liu Lixin, Tao Ye, Ren Zhongjuan
机构信息
Department of Preventive Medicine, School of Public Health, Jiamusi University, Jiamusi, Heilongjiang 154007, P.R. China.
Department of Animal Science, College of Life Science, Jiamusi University, Jiamusi, Heilongjiang 154007, P.R. China.
出版信息
Exp Ther Med. 2017 Aug;14(2):1795-1801. doi: 10.3892/etm.2017.4638. Epub 2017 Jun 21.
Prostate stem cell antigen (PSCA) is expressed in the majority of prostate cancer cases and may be a potential therapeutic target in the treatment of prostate cancer. The present study evaluated the cytotoxicity of three flavonoids (genistein, luteolin and quercetin) towards DU145 prostate cancer cells, and investigated the effect of these flavonoids on PSCA expression. The results demonstrated that genistein, luteolin and quercetin inhibited the growth of DU145 cells in a dose-dependent manner (P<0.05) and induced morphological changes characteristic of apoptosis in DU145 cells. Flow cytometry analysis also indicated that the flavonoids induced S phase cycle arrest in DU145 cells. Notably, it was observed that expression of PSCA was inhibited at the mRNA (P<0.05) and protein levels in DU145 cells following flavonoid treatment compared with the control. These results suggest that flavonoids may be potential therapeutic agents in the treatment and prevention of prostate cancer.
前列腺干细胞抗原（PSCA）在大多数前列腺癌病例中表达，可能是前列腺癌治疗中的一个潜在治疗靶点。本研究评估了三种黄酮类化合物（染料木黄酮、木犀草素和槲皮素）对DU145前列腺癌细胞的细胞毒性，并研究了这些黄酮类化合物对PSCA表达的影响。结果表明，染料木黄酮、木犀草素和槲皮素以剂量依赖性方式抑制DU145细胞的生长（P<0.05），并诱导DU145细胞出现凋亡特征性的形态变化。流式细胞术分析还表明，这些黄酮类化合物诱导DU145细胞S期周期阻滞。值得注意的是，观察到与对照组相比，黄酮类化合物处理后DU145细胞中PSCA的mRNA（P<0.05）和蛋白质水平表达受到抑制。这些结果表明，黄酮类化合物可能是治疗和预防前列腺癌的潜在治疗药物。
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