Ritzer J, Lühmann T, Rode C, Pein-Hackelbusch M, Immohr I, Schedler U, Thiele T, Stübinger S, Rechenberg B V, Waser-Althaus J, Schlottig F, Merli M, Dawe H, Karpíšek M, Wyrwa R, Schnabelrauch M, Meinel L
Institute for Pharmacy and Food Chemistry, Universität Würzburg, Am Hubland, 97074, Würzburg, Germany.
Biomaterials Department, Innovent e.V., Prüssingstraße 27B, 07745, Jena, Germany.
Nat Commun. 2017 Aug 15;8(1):264. doi: 10.1038/s41467-017-00340-x.
Our ability of screening broad communities for clinically asymptomatic diseases critically drives population health. Sensory chewing gums are presented targeting the tongue as 24/7 detector allowing diagnosis by "anyone, anywhere, anytime". The chewing gum contains peptide sensors consisting of a protease cleavable linker in between a bitter substance and a microparticle. Matrix metalloproteinases in the oral cavity, as upregulated in peri-implant disease, specifically target the protease cleavable linker while chewing the gum, thereby generating bitterness for detection by the tongue. The peptide sensors prove significant success in discriminating saliva collected from patients with peri-implant disease versus clinically asymptomatic volunteers. Superior outcome is demonstrated over commercially available protease-based tests in saliva. "Anyone, anywhere, anytime" diagnostics are within reach for oral inflammation. Expanding this platform technology to other diseases in the future features this diagnostic as a massive screening tool potentially maximizing impact on population health.Early detection of gum inflammation caused by dental implants helps prevent tissue damage. Here, the authors present a peptide sensor that generates a bitter taste when cleaved by proteases present in peri-implant disease, embed it in a chewing gum, and compare the probe to existing sensors using patient saliva.
我们在广泛人群中筛查临床无症状疾病的能力对人群健康至关重要。本文介绍了一种针对舌头的感官口香糖，它就像一个全天候的探测器，能让“任何人在任何地点、任何时间”进行诊断。这种口香糖含有肽传感器，由苦味物质和微粒之间的蛋白酶可裂解连接子组成。在种植体周围疾病中上调的口腔基质金属蛋白酶，在咀嚼口香糖时会特异性地作用于蛋白酶可裂解连接子，从而产生苦味供舌头检测。肽传感器在区分种植体周围疾病患者与临床无症状志愿者的唾液方面取得了显著成功。与市售的基于蛋白酶的唾液检测方法相比，结果更优。“任何人在任何地点、任何时间”进行口腔炎症诊断已指日可待。未来将这种平台技术扩展到其他疾病，有望使这种诊断成为一种大规模筛查工具，对人群健康产生最大影响。早期检测由牙种植体引起的牙龈炎症有助于预防组织损伤。在此，作者展示了一种肽传感器，当被种植体周围疾病中存在的蛋白酶裂解时会产生苦味，将其嵌入口香糖中，并使用患者唾液将该探针与现有传感器进行比较。
