Department of Oral and Maxillofacial Diseases, Helsinki University Hospital, University of Helsinki, Helsinki, Finland.
Department of Dental Medicine, Division of Periodontology, Karolinska Institutet, Stockholm, Sweden.
Dis Markers. 2018 Aug 5;2018:1306396. doi: 10.1155/2018/1306396. eCollection 2018.
The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth rinse, gingival crevicular fluid (GCF), and peri-implantitis fluid (PISF)) is demanding. Several hosts and microbial factors may influence their expression, release, and levels. The type of saliva/oral fluids utilized for the diagnostics affects the analysis. High sensitivity and specificities together with sophisticated methods and techniques are essential for valuable outcome. We describe here recently developed practical, convenient, inexpensive, noninvasive, and quantitative mouth rinse and PISF/GCF/chair-side/point-of-care (PoC) lateral-flow aMMP-8 immunoassays (PerioSafe and ImplantSafe/ORALyser) to detect, predict, and monitor successfully the course, treatment, and prevention of periodontitis and peri-implantitis, respectively. The tests have been independently and successfully validated to differentiate periodontal and peri-implant health and disease in Finland, Germany, Netherland, Sweden, Turkey, Nigeria, Malawi, and USA. The clinical use of salivary/oral fluid biomarkers to identify oral and systemic conditions requires additional studies utilizing these noninvasive screening, diagnostic, and preventive aMMP-8 PoC/chair-side technologies.
分析唾液和口腔液（即漱口液、龈沟液 (GCF) 和种植体周围炎液 (PISF)）中的疾病特异性口腔和全身生物标志物具有一定难度。多种宿主和微生物因素可能影响它们的表达、释放和水平。用于诊断的唾液/口腔液类型会影响分析结果。高灵敏度和特异性以及复杂的方法和技术对于获得有价值的结果至关重要。我们在这里描述了最近开发的实用、方便、经济、非侵入性和定量的漱口液和 PISF/GCF/椅旁/即时检测 (PoC) 基质金属蛋白酶-8 (MMP-8) 免疫测定法 (PerioSafe 和 ImplantSafe/ORALyser)，以成功地检测、预测和监测牙周炎和种植体周围炎的病程、治疗和预防。这些测试已在芬兰、德国、荷兰、瑞典、土耳其、尼日利亚、马拉维和美国进行了独立和成功的验证，可用于区分牙周炎和种植体周围炎的健康和疾病。唾液/口腔液生物标志物在口腔和全身疾病中的临床应用需要进一步研究，以利用这些非侵入性的筛查、诊断和预防 MMP-8 PoC/椅旁技术。
