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趋磁细菌中局部铁积累先于磁铁矿晶体的成核和生长。

Localized iron accumulation precedes nucleation and growth of magnetite crystals in magnetotactic bacteria.

机构信息

Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil.

Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil.

出版信息

Sci Rep. 2017 Aug 15;7(1):8291. doi: 10.1038/s41598-017-08994-9.

DOI:10.1038/s41598-017-08994-9
PMID:28811607
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5557804/
Abstract

Many magnetotactic bacteria (MTB) biomineralize magnetite crystals that nucleate and grow inside intracellular membranous vesicles that originate from invaginations of the cytoplasmic membrane. The crystals together with their surrounding membranes are referred to magnetosomes. Magnetosome magnetite crystals nucleate and grow using iron transported inside the vesicle by specific proteins. Here we address the question: can iron transported inside MTB for the production of magnetite crystals be spatially mapped using electron microscopy? Cultured and uncultured MTB from brackish and freshwater lagoons were studied using analytical transmission electron microscopy in an attempt to answer this question. Scanning transmission electron microscopy was used at sub-nanometric resolution to determine the distribution of elements by implementing high sensitivity energy dispersive X-ray (EDS) mapping and electron energy loss spectroscopy (EELS). EDS mapping showed that magnetosomes are enmeshed in a magnetosomal matrix in which iron accumulates close to the magnetosome forming a continuous layer visually appearing as a corona. EELS, obtained at high spatial resolution, confirmed that iron was present close to and inside the lipid bilayer magnetosome membrane. This study provides important clues to magnetite formation in MTB through the discovery of a mechanism where iron ions accumulate prior to magnetite biomineralization.

摘要

许多磁细菌（MTB）将磁铁矿晶体生物矿化，这些晶体在内质膜内陷形成的细胞内膜状小泡中形成并生长。这些晶体与周围的膜一起被称为磁小体。磁小体磁铁矿晶体的成核和生长是利用特定蛋白质在囊泡内运输的铁来实现的。在这里，我们提出了一个问题：能否使用电子显微镜对用于磁铁矿晶体生产的 MTB 内部运输的铁进行空间定位？本研究使用分析型透射电子显微镜对来自咸水和淡水泻湖的培养和未培养的 MTB 进行了研究，试图回答这个问题。通过实施高灵敏度能量色散 X 射线（EDS）映射和电子能量损失光谱（EELS），扫描透射电子显微镜以亚纳米分辨率用于确定元素的分布。EDS 映射表明，磁小体被包裹在一个磁小体基质中，铁在靠近磁小体的地方积累，形成一层连续的层，在视觉上呈现出冠状。在高空间分辨率下获得的 EELS 证实，铁存在于靠近和内部的脂质双层磁小体膜中。这项研究通过发现一种在磁铁矿生物矿化之前铁离子积累的机制，为 MTB 中的磁铁矿形成提供了重要线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/162d462b3889/41598_2017_8994_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/bde1943bc510/41598_2017_8994_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/3bcdfc39d52e/41598_2017_8994_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/af042ce6ce74/41598_2017_8994_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/c0db6d8be5ed/41598_2017_8994_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/3d05a5e12cf3/41598_2017_8994_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/5109a456716b/41598_2017_8994_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/162d462b3889/41598_2017_8994_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/bde1943bc510/41598_2017_8994_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/3bcdfc39d52e/41598_2017_8994_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/af042ce6ce74/41598_2017_8994_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/c0db6d8be5ed/41598_2017_8994_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/3d05a5e12cf3/41598_2017_8994_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/5109a456716b/41598_2017_8994_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e386/5557804/162d462b3889/41598_2017_8994_Fig7_HTML.jpg

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