Eid Neveen H, Al Doghaither Huda A, Kumosani Taha A, Gull Munazza
Neveen H. Eid, Biochemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 42805, Jeddah, Saudi Arabia.
Dr. Huda A. Al Doghaither, Biochemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 42805, Jeddah, Saudi Arabia.
Pak J Med Sci. 2017 May-Jun;33(3):703-708. doi: 10.12669/pjms.333.12347.
To evaluate the indigenous bacterial strains of drinking water from the most commercial water types including bottled and filtered water that are currently used in Saudi Arabia.
Thirty randomly selected commercial brands of bottled water were purchased from Saudi local markets. Moreover, samples from tap water and filtered water were collected in sterilized glass bottles and stored at 4°C. Biochemical analyses including pH, temperature, lactose fermentation test (LAC), indole test (IND), methyl red test (MR), Voges-Proskauer test (VP), urease test (URE), catalase test (CAT), aerobic and anaerobic test (Ae/An) were measured. Molecular identification and comparative sequence analyses were done by full length 16S rRNA gene sequences using gene bank databases and phylogenetic trees were constructed to see the closely related similarity index between bacterial strains.
Among 30 water samples tested, 18 were found positive for bacterial growth. Molecular identification of four selected bacterial strains indicated the alarming presence of pathogenic bacteria . in most common commercial types of drinking water used in Saudi Arabia.
The lack of awareness about good sanitation, poor personal hygienic practices and failure of safe water management and supply are the important factors for poor drinking water quality in these sources, need to be addressed.
评估沙特阿拉伯目前使用的包括瓶装水和过滤水在内的最常见商业类型饮用水中的本土细菌菌株。
从沙特当地市场随机购买30个商业品牌的瓶装水。此外，从自来水和过滤水中采集的样本保存在无菌玻璃瓶中，并在4°C下储存。进行了包括pH值、温度、乳糖发酵试验（LAC）、吲哚试验（IND）、甲基红试验（MR）、Voges-Proskauer试验（VP）、脲酶试验（URE）、过氧化氢酶试验（CAT）、需氧和厌氧试验（Ae/An）在内的生化分析。通过使用基因库数据库的全长16S rRNA基因序列进行分子鉴定和比较序列分析，并构建系统发育树以查看细菌菌株之间的密切相关相似性指数。
在测试的30个水样中，有18个被发现细菌生长呈阳性。对四种选定细菌菌株的分子鉴定表明，在沙特阿拉伯使用的最常见商业类型饮用水中存在令人担忧的致病细菌。
对良好卫生条件缺乏认识、个人卫生习惯差以及安全水管理和供应失败是这些水源饮用水质量差的重要因素，需要加以解决。
