Qureshi Naeela, Bariana Harbans, Kolmer James A, Miah Hanif, Bansal Urmil
First, second, fourth, and fifth authors: The University of Sydney Plant Breeding Institute, Private Bag 4011, Narellan, NSW 2567, Australia; and third author: United States Department of Agriculture-Agricultural Research Service Cereal Disease Laboratory, St. Paul, MN, 55108.
Phytopathology. 2017 Nov;107(11):1381-1387. doi: 10.1094/PHYTO-01-17-0005-R. Epub 2017 Aug 16.
Leaf rust, caused by Puccinia triticina, is a constraint to durum wheat (Triticum turgidum subsp. durum) production, and landraces are reported to be an important source of resistance. Two Portuguese landraces (Aus26582 and Aus26579) showed resistance against durum-specific P. triticina races and were crossed with a susceptible landrace (Bansi) to develop recombinant inbred line (RIL) populations. Monogenic segregation for leaf rust resistance was observed among both RIL populations. The underlying locus, temporarily named LrAW2, was mapped to the short arm of chromosome 6B in the Aus26582/Bansi population and five DArTseq markers cosegregated with LrAW2. Simple sequence repeat markers sun683 and sun684, developed from the chromosome survey sequence (CSS) contig 6BS_2963854, identified through BlastN search of cosegregating DArTseq markers in the International Wheat Genome Sequencing Consortium database, cosegregated with LrAW2. Comparison of the CSS contig 6BS_2963854-based sequences amplified from parental genotypes led to the development of marker sunKASP_60, which also showed close linkage with LrAW2. Markers sun684 and sunKASP_60 showed close association with LrAW2 in both RIL populations. The amplification of LrAW2-specific products by linked markers in Aus26582, Aus26579, and Guayacan (Lr61) indicated that LrAW2 may be Lr61. The alternate amplicon or haplotype produced with LrAW2-linked markers in Australian durum cultivars demonstrated their effectiveness in marker-assisted selection.
由小麦条锈菌（Puccinia triticina）引起的叶锈病是硬粒小麦（Triticum turgidum subsp. durum）生产的一个限制因素，据报道地方品种是重要的抗性来源。两个葡萄牙地方品种（Aus26582和Aus26579）对硬粒小麦特有的小麦条锈菌生理小种表现出抗性，并与一个感病地方品种（Bansi）杂交，以构建重组自交系（RIL）群体。在两个RIL群体中均观察到叶锈病抗性的单基因分离。潜在的基因座暂时命名为LrAW2，在Aus26582/Bansi群体中被定位到6B染色体的短臂上，并且五个DArTseq标记与LrAW2共分离。通过在国际小麦基因组测序联盟数据库中对共分离的DArTseq标记进行BlastN搜索鉴定出的、基于染色体调查序列（CSS）重叠群6BS_2963854开发的简单序列重复标记sun683和sun684与LrAW2共分离。对从亲本基因型扩增的基于CSS重叠群6BS_2963854的序列进行比较，开发出了标记sunKASP_60，其也与LrAW2紧密连锁。标记sun684和sunKASP_60在两个RIL群体中均与LrAW2紧密关联。在Aus26582、Aus26579和Guayacan（Lr61）中，与LrAW2连锁的标记对LrAW2特异性产物的扩增表明LrAW2可能是Lr61。在澳大利亚硬粒小麦品种中，由与LrAW2连锁的标记产生的替代扩增子或单倍型证明了它们在标记辅助选择中的有效性。
