Bulman Sophie E L, Tronci Giuseppe, Goswami Parikshit, Carr Chris, Russell Stephen J
Clothworkers' Centre for Textile Materials Innovation for Healthcare (CCTMIH), School of Design, University of Leeds, Leeds LS2 9JT, West Yorkshire, UK.
Materials (Basel). 2017 Aug 16;10(8):954. doi: 10.3390/ma10080954.
Manuka honey (MH) is used as an antibacterial agent in bioactive wound dressings via direct impregnation onto a suitable substrate. MH provides unique antibacterial activity when compared with conventional honeys, owing partly to one of its constituents, methylglyoxal (MGO). Aiming to investigate an antibiotic-free antimicrobial strategy, we studied the antibacterial activity of both MH and MGO (at equivalent MGO concentrations) when applied as a physical coating to a nonwoven fabric wound dressing. When physically coated on to a cellulosic hydroentangled nonwoven fabric, it was found that concentrations of 0.0054 mg cm of MGO in the form of MH and MGO were sufficient to achieve a 100 colony forming unit % bacteria reduction against gram-positive and gram-negative , based on BS EN ISO 20743:2007. A 3- to 20-fold increase in MGO concentration (0.0170-0.1 mg cm) was required to facilitate a good antibacterial effect (based on BS EN ISO 20645:2004) in terms of zone of inhibition and lack of growth under the sample. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was also assessed for MGO in liquid form against three prevalent wound and healthcare-associated pathogens, i.e., , gram-negative and gram-positive . Other than the case of MGO-containing fabrics, solutions with much higher MGO concentrations (128 mg L-1024 mg L) were required to provide either a bacteriostatic or bactericidal effect. The results presented in this study therefore demonstrate the relevance of an MGO-based coating as an environmentally friendly strategy for the design of functional dressings with antibiotic-free antimicrobial chemistries.
麦卢卡蜂蜜（MH）通过直接浸渍到合适的基质上，作为生物活性伤口敷料中的抗菌剂。与传统蜂蜜相比，MH具有独特的抗菌活性，部分原因在于其成分之一甲基乙二醛（MGO）。为了研究一种无抗生素的抗菌策略，我们研究了将MH和MGO（在等效MGO浓度下）作为物理涂层应用于无纺布伤口敷料时的抗菌活性。当物理涂覆在纤维素水刺无纺布上时，发现以MH和MGO形式存在的0.0054 mg/cm的MGO浓度足以根据BS EN ISO 20743:2007实现对革兰氏阳性菌和革兰氏阴性菌100个菌落形成单位%的细菌减少。为了在抑制圈和样品下无生长方面促进良好的抗菌效果（基于BS EN ISO 20645:2004），需要将MGO浓度提高3至20倍（0.0170 - 0.1 mg/cm）。还评估了液体形式的MGO对三种常见伤口和医疗保健相关病原体，即革兰氏阴性菌和革兰氏阳性菌的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。除了含MGO的织物情况外，需要更高MGO浓度（128 mg/L - 1024 mg/L）的溶液才能提供抑菌或杀菌效果。因此，本研究中呈现的结果证明了基于MGO的涂层作为一种环保策略在设计具有无抗生素抗菌化学的功能性敷料方面的相关性。
