甲基乙二醛：对伤寒沙门氏菌及其他革兰氏阴性杆菌的血培养分离株的抗菌活性。
Methylglyoxal: Antimicrobial activity against blood culture isolates of Salmonella Typhi and other Gram negative rods.
作者信息
Afzal Raja Kamran, Khalid Fizza, Hannan Abdul, Ahmed Syed Azhar
机构信息
Dr. Raja Kamran Afzal, FCPS, PhD. Department of Pathology, Armed Forces Institute of Cardiology, Rawalpindi, Pakistan.
Fizza Khalid, Department of Pathology, University of Health Sciences, Lahore, Pakistan.
出版信息
Pak J Med Sci. 2019 Jul-Aug;35(4):1110-1114. doi: 10.12669/pjms.35.4.807.
OBJECTIVES
To evaluate the activity of Methylglyoxal against the blood culture isolates of Salmonella Typhi and various Gram negative rods and to compare the activity of Methylglyoxal against S. Typhi and other Gram negative rods.
METHODS
It was an experimental study conducted at the Department of Microbiology, University of Health Sciences (UHS), Lahore-Pakistan in collaboration with the Department of Microbiology, CMH Lahore, from July 2011 to June 2012. Recent blood culture isolates of and other Gram negative rods were collected from different hospitals of Lahore and kept stored at -80°C. As per the latest CLSI guidelines, morphological, biochemical and serological identification was carried out and antimicrobial susceptibility was tested. A multi-point inoculator was used to carry out agar dilution for determination of MICs of MGO. Results were determined after compilation of data using latest SPSS version.
RESULTS
MIC of MGO against the clinical isolates of S. Typhi was 0.20 (2.8 ) and against Gram negative rods it was 0.21 (3.0 ). The p-value of MICs of MGO against the isolates of S. Typhi was 0.023 when compared with Gram negative rods (p<0.05; statistically significant).
CONCLUSION
MGO has a scientifically proven antimicrobial activity against blood culture isolates of S. Typhi and various Gram negative rods.
目的
评估甲基乙二醛对伤寒沙门菌血培养分离株及各种革兰氏阴性杆菌的活性，并比较甲基乙二醛对伤寒沙门菌和其他革兰氏阴性杆菌的活性。
方法
这是一项于2011年7月至2012年6月在巴基斯坦拉合尔健康科学大学（UHS）微生物学系与拉合尔CMH微生物学系合作开展的实验研究。从拉合尔不同医院收集近期的伤寒沙门菌及其他革兰氏阴性杆菌血培养分离株，并保存在-80°C。按照最新的CLSI指南，进行形态学、生化和血清学鉴定，并检测抗菌药敏性。使用多点接种仪进行琼脂稀释法测定甲基乙二醛的最低抑菌浓度（MIC）。使用最新版SPSS软件汇总数据后得出结果。
结果
甲基乙二醛对伤寒沙门菌临床分离株的MIC为0.20 （2.8 ），对革兰氏阴性杆菌的MIC为0.21 （3.0 ）。与革兰氏阴性杆菌相比，甲基乙二醛对伤寒沙门菌分离株的MIC的p值为0.023（p<0.05；具有统计学意义）。
结论
甲基乙二醛对伤寒沙门菌血培养分离株及各种革兰氏阴性杆菌具有经科学验证的抗菌活性。
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