Hayes G, Wright N, Gardner S L, Telzrow C L, Wommack A J, Vigueira P A
Department of Biology, High Point University, High Point, NC, USA.
Department of Chemistry, High Point University, High Point, NC, USA.
Lett Appl Microbiol. 2018 Jun;66(6):491-495. doi: 10.1111/lam.12880. Epub 2018 Apr 19.
The continued emergence and global spread of bacterial antibiotic resistance has fuelled the search for novel antimicrobial agents and resistance-modifying compounds. Manuka honey has both antimicrobial properties and the ability to increase the efficacy of FDA-approved antibiotic drugs. Compared to other types of honey, manuka honey contains elevated levels of methylglyoxal (MGO), a small molecule that contributes to its antibacterial activity. Manuka honey has shown particular promise for the treatment of antibiotic-resistant Gram-positive organisms such as methicillin-resistant Staphylococcus aureus. Linezolid is an oxazolidinone antibiotic used in the treatment of infections caused by a range of Gram-positive pathogens. Here, we demonstrate that manuka honey, as well as MGO in isolation, increases the sensitivity of S. aureus to linezolid in both agar diffusion and broth microdilution assays. This synergistic interaction is mediated in part by increased intracellular accumulation of linezolid in the presence of MGO.
Manuka honey is widely recognized for its antimicrobial activity. Our study adds to the growing body of evidence that manuka honey and its active ingredient, methylglyoxal (MGO), can also function as antibiotic adjuvants. In this study, we provide the first report of synergy between MGO and linezolid against Staphylococcus aureus. Both manuka honey and purified MGO significantly increased the sensitivity of S. aureus to linezolid.
细菌对抗生素耐药性的持续出现和全球传播推动了对新型抗菌剂和耐药性修饰化合物的研究。麦卢卡蜂蜜具有抗菌特性，并且能够提高美国食品药品监督管理局（FDA）批准的抗生素药物的疗效。与其他类型的蜂蜜相比，麦卢卡蜂蜜含有更高水平的甲基乙二醛（MGO），这种小分子有助于其抗菌活性。麦卢卡蜂蜜在治疗耐抗生素的革兰氏阳性菌（如耐甲氧西林金黄色葡萄球菌）方面显示出特别的前景。利奈唑胺是一种恶唑烷酮类抗生素，用于治疗由多种革兰氏阳性病原体引起的感染。在此，我们证明，在琼脂扩散试验和肉汤微量稀释试验中，麦卢卡蜂蜜以及单独的MGO均可提高金黄色葡萄球菌对利奈唑胺的敏感性。这种协同相互作用部分是由在MGO存在下利奈唑胺细胞内积累增加所介导的。
麦卢卡蜂蜜因其抗菌活性而被广泛认可。我们的研究进一步增加了越来越多的证据，表明麦卢卡蜂蜜及其活性成分甲基乙二醛（MGO）也可作为抗生素佐剂发挥作用。在本研究中，我们首次报道了MGO与利奈唑胺对金黄色葡萄球菌的协同作用。麦卢卡蜂蜜和纯化的MGO均显著提高了金黄色葡萄球菌对利奈唑胺的敏感性。
