Bondan Eduardo, Cardoso Carolina, Martins Maria de Fátima
Universidade Paulista, Patologia Ambiental e Experimental, São Paulo SP, Brasil.
Universidade Cruzeiro do Sul, Medicina Veterinária, São Paulo SP, Brasil.
Arq Neuropsiquiatr. 2017 Aug;75(8):546-552. doi: 10.1590/0004-282X20170092.
Recent studies have demonstrated that curcumin (Cur) has antioxidant, anti-inflammatory and anti-fibrotic effects. Ethidium bromide (EB) injections into the central nervous system (CNS) are known to induce local oligodendroglial and astrocytic loss, resulting in primary demyelination and neuroinflammation. Peripheral astrogliosis is seen around the injury site with increased immunoreactivity to glial fibrillary acidic protein (GFAP). This investigation aimed to evaluate the effect of Cur administration on astrocytic response following gliotoxic injury. Wistar rats were injected with EB into the cisterna pontis and treated, or not, with Cur (100 mg/kg/day, intraperitoneal route) during the experimental period. Brainstem sections were collected at 15, 21 and 31 days after EB injection and processed for GFAP immunohistochemical staining. Astrocytic reactivity was measured in a computerized system for image analysis. In Cur-treated rats, the GFAP-stained area around the lesion was significantly smaller in all periods after EB injection compared to untreated animals, showing that Cur reduces glial scar development following injury.
最近的研究表明，姜黄素（Cur）具有抗氧化、抗炎和抗纤维化作用。已知向中枢神经系统（CNS）注射溴化乙锭（EB）会导致局部少突胶质细胞和星形胶质细胞丢失，从而导致原发性脱髓鞘和神经炎症。在损伤部位周围可见外周星形胶质细胞增生，对胶质纤维酸性蛋白（GFAP）的免疫反应性增加。本研究旨在评估给予Cur对神经毒性损伤后星形胶质细胞反应的影响。在实验期间，将Wistar大鼠脑桥池注射EB，并给予或不给予Cur（100mg/kg/天，腹腔注射途径）。在注射EB后15、21和31天收集脑干切片，并进行GFAP免疫组织化学染色。在计算机图像分析系统中测量星形胶质细胞反应性。与未治疗的动物相比，在Cur治疗的大鼠中，EB注射后所有时期损伤周围GFAP染色区域明显更小，表明Cur可减少损伤后胶质瘢痕的形成。
