Bondan Eduardo Fernandes, Lallo Maria Anete, Dagli Maria Lúcia Zaidan, Pereira Luís Antonio Violin Dias, Graca Dominguita Luhers
Universidade Paulista, São Paulo, SP, Brasil.
Arq Neuropsiquiatr. 2002 Sep;60(3-A):582-9.
Ethidium bromide (EB) causes local astrocytic disappearance, with glia limitans disruption and supposed blood-brain barrier (BBB) breakdown The aim of this study was to investigate the BBB integrity after the injection of 0.1% EB (group E) or 0.9% saline solution (group C) into cisterna pontis of Wistar rats. Brainstem fragments were collected from 24 hours to 31 days post-injection for ultrastructural study and GFAP immuno-histochemical staining. Some animals received colloidal carbon ink by intravenous route at the same periods. In rats from group C, there was no sign of astrocyte loss and no leakage of ink from blood vessels in the injection site. In group E, astrocyte disappearance began at 48 hours and some areas were still devoid of astrocytic processes 31 days after. Leakage of carbon particles was seen from 48 hours to 7 days in the EB-induced lesions. Tight junctions did not show any detectable ultrastructural change due to the lack of perivascular astrocytes.
溴化乙锭（EB）会导致局部星形胶质细胞消失，伴有胶质界膜破坏以及推测的血脑屏障（BBB）破坏。本研究的目的是在向Wistar大鼠脑桥池注射0.1% EB（E组）或0.9%盐溶液（C组）后，研究血脑屏障的完整性。在注射后24小时至31天收集脑干碎片用于超微结构研究和GFAP免疫组织化学染色。在同一时期，一些动物通过静脉途径接受胶体碳墨汁。在C组大鼠中，没有星形胶质细胞丢失的迹象，且注射部位血管没有墨汁渗漏。在E组中，星形胶质细胞在48小时开始消失，31天后一些区域仍没有星形胶质细胞突起。在EB诱导的损伤部位，从48小时至7天可见碳颗粒渗漏。由于缺乏血管周围星形胶质细胞，紧密连接未显示任何可检测到的超微结构变化。
