Bondan Eduardo Fernandes, Lallo Maria Anete, Dagli Maria Lúcia Zaidan, Sanchez Melissa, Graça Dominguita Lühers
Universidade Bandeirante de São Paulo, São Paulo, SP, Brasil.
Arq Neuropsiquiatr. 2003 Sep;61(3A):642-9. doi: 10.1590/s0004-282x2003000400022. Epub 2003 Sep 16.
Ethidium bromide (EB) is known as a gliotoxic agent that causes focal astrocytic and oligodendroglial disappearance.
Astrocyte immunoreactivity to glial fibrillary acidic protein (GFAP) and vimentin (VIM) was investigated after EB injection.
Adult male Wistar rats were taken as histologic controls (group H) or injected into cisterna pontis with 0.1% EB (group E) or 0.9% saline solution (group C). Brainstem samples were collected from 24 hours to 31 days post-injection for GFAP and VIM immunohistochemical staining using avidin-biotin method.
In group E, extensive lesions were seen in the pons and mesencephalon, with astrocyte disappearance from the central area 24 hours post-injection. Macrophagic infiltration and peripheral astrocytic reaction were noted after 3 days. Marginal astrocytes presented increased immunoreactivity to GFAP and reexpression of VIM, the last one confined to the edges of the injury site. In group C, discrete pontine lesions were observed, showing central astrocyte preservation and a peripheral GFAP staining less intense comparing to group E. No immunoreactivity to VIM was noted in such astrocytes.
Astrocytes from the edges of the EB-induced lesions presented increased immunoreactivity to GFAP and reexpression of VIM.
溴化乙锭（EB）是一种已知的神经胶质毒性剂，可导致局灶性星形胶质细胞和少突胶质细胞消失。
研究EB注射后星形胶质细胞对胶质纤维酸性蛋白（GFAP）和波形蛋白（VIM）的免疫反应性。
将成年雄性Wistar大鼠作为组织学对照（H组），或向脑桥池注射0.1%EB（E组）或0.9%盐溶液（C组）。在注射后24小时至31天收集脑干样本，采用抗生物素蛋白-生物素方法进行GFAP和VIM免疫组织化学染色。
在E组中，脑桥和中脑出现广泛病变，注射后24小时中央区域星形胶质细胞消失。3天后可见巨噬细胞浸润和外周星形胶质细胞反应。边缘星形胶质细胞对GFAP的免疫反应性增加，VIM重新表达，后者局限于损伤部位边缘。在C组中，观察到离散的脑桥病变，显示中央星形胶质细胞保存，与E组相比外周GFAP染色较弱。此类星形胶质细胞对VIM无免疫反应性。
EB诱导病变边缘的星形胶质细胞对GFAP的免疫反应性增加，VIM重新表达。
