Bondan Eduardo Fernandes, Martins Maria de Fátima Monteiro, Viani Flávio Cesar
Arq Bras Endocrinol Metabol. 2013 Aug;57(6):431-6. doi: 10.1590/s0004-27302013000600004.
The aim of this study was to evaluate the effect of diabetic hyperglycemia on astrocyte function, estimated by means of glial fibrillary acidic protein - GFAP - immunohistochemical expression.
Adult male rats received a single intravenous injection of streptozotocin (50 mg/kg) and were submitted 10 days later to a single injection of 10 microlitres 0.1% EB solution or 0.9% saline solution into the cisterna pontis. Ten microliters of 0.1% EB or 0.9% saline solution were also injected in non-diabetic rats. Animals were anesthetized and perfused through the heart 15 and 31 days after EB or saline injection, and brainstem sections were collected for ultrastructural analysis and GFAP immunohistochemical staining.
The GFAP brown-stained areas were evaluated by colorimetry using a computerized image analysis system and the results have shown that diabetes hindered the increase of GFAP astrocyte expression in the EB-injected group compared to non-diabetic animals. However, diabetes did not affect GFAP response in the saline-injected group or in control animals.
Streptozotocin-induced diabetic condition reduced astrocytic GFAP expression following gliotoxic injury.
本研究旨在通过胶质纤维酸性蛋白（GFAP）免疫组化表达评估糖尿病高血糖对星形胶质细胞功能的影响。
成年雄性大鼠单次静脉注射链脲佐菌素（50mg/kg），10天后于脑桥池注射10微升0.1%伊文思蓝（EB）溶液或0.9%盐溶液。非糖尿病大鼠也注射10微升0.1%EB或0.9%盐溶液。在注射EB或盐溶液后15天和31天，将动物麻醉并经心脏灌注，收集脑干切片进行超微结构分析和GFAP免疫组化染色。
使用计算机图像分析系统通过比色法评估GFAP棕色染色区域，结果显示，与非糖尿病动物相比，糖尿病阻碍了注射EB组中GFAP星形胶质细胞表达的增加。然而，糖尿病对注射盐溶液组或对照动物的GFAP反应没有影响。
链脲佐菌素诱导的糖尿病状态在胶质毒性损伤后降低了星形胶质细胞GFAP表达。
