Masuoka Hiroaki, Shimada Kouya, Kiyosue-Yasuda Tomoyo, Kiyosue Masaharu, Oishi Yukie, Kimura Seiji, Ohashi Yuji, Fujisawa Tomohiko, Hotta Kozue, Yamada Akio, Hirayama Kazuhiro
Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
Nisshin Petfood Inc., Chiyoda-ku, Tokyo, Japan.
PLoS One. 2017 Aug 16;12(8):e0181739. doi: 10.1371/journal.pone.0181739. eCollection 2017.
The transition of intestinal microbiota with age has been well described in humans. However, the age-related changes in intestinal microbiota of cats have not been well studied. In the present study, we investigated the composition of intestinal microbiota of cats in 5 different age groups (pre-weanling, weanling, young, aged, senile) with a culture-based method. For lactobacilli and bifidobacteria, we also quantified with molecular-based method, real-time PCR. The results suggested that the composition of the feline intestinal microbiota changes with age, while the changes were different from those of humans and dogs. Bifidobacteria which are predominant in human intestine or lactobacilli which are predominant in dog intestine, did not appear to be important in cat intestines. Enterococci, instead, seem to be major lactic acid producing bacteria in cats. We also identified lactobacilli and bifidobacteria at the species level based on 16S rRNA gene sequences and found that the species composition of Lactobacillus also changed with age.
肠道微生物群随年龄的变化在人类中已有充分描述。然而，猫肠道微生物群与年龄相关的变化尚未得到充分研究。在本研究中，我们采用基于培养的方法调查了5个不同年龄组（断奶前、断奶期、幼年、成年、老年）猫的肠道微生物群组成。对于乳酸菌和双歧杆菌，我们还采用基于分子的方法——实时定量聚合酶链反应进行了定量分析。结果表明，猫肠道微生物群的组成随年龄变化，而这些变化与人类和狗不同。在人类肠道中占主导地位的双歧杆菌或在狗肠道中占主导地位的乳酸菌，在猫肠道中似乎并不重要。相反，肠球菌似乎是猫体内主要的产乳酸菌。我们还基于16S rRNA基因序列在种水平上鉴定了乳酸菌和双歧杆菌，发现乳酸菌的物种组成也随年龄变化。
