Bashandy Samir A E, Alaamer Abdulaziz, Moussa Sherif A Abdelmottaleb, Omara Enayat A
a Department of Pharmacology, Medical Division, National Research Centre, 33 EL Bohouth St. (former EL Tahir St.), Dokki, Giza, Egypt. P.O.12622.
b Committee of Radiation and Environmental Pollution Protection (CREPP), Department of Physics, College of Science, Al-Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, Saudi Arabia.
Can J Physiol Pharmacol. 2018 Apr;96(4):337-344. doi: 10.1139/cjpp-2017-0247. Epub 2017 Aug 16.
The present research studied the influence of zinc oxide nanoparticles (ZnO-NPs; 5, 7.5, and 10 mg/kg, i.p.) on the liver and kidney injuries motivated by thioacetamide (TAA; 100 mg/kg, i.p.). Each treatment was carried out 3 times per week for 8 weeks. ZnO-NPs relieved the decrease of hepatic or renal reduced glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) induced by TAA. Moreover, ZnO-NPs lowered tissue malondialdehyde (MDA, an indicator for lipid peroxidation). TAA treatment led to a significant increase in plasma inflammatory markers (TNF-α, IL-6), liver enzymes (gamma-glutamyltransferase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and kidney function parameters (creatinine, urea, uric acid). However, these parameters were reduced after treatment with ZnO-NPs. In addition, the hepatic fibrosis markers, hydroxyproline level, and α-smooth muscle actin immunopositive stain were lowered by ZnO-NPs. The protective effect of ZnO-NPs in respect to biochemical changes was also confirmed by histopathological and immunohistochemistry studies in the liver and kidney sections. Our results suggested that ZnO-NPs may attenuate TAA toxicity via suppression of oxidative stress.
本研究探讨了纳米氧化锌（ZnO-NPs；腹腔注射剂量为5、7.5和10 mg/kg）对硫代乙酰胺（TAA；腹腔注射剂量为100 mg/kg）所致肝损伤和肾损伤的影响。每周进行3次治疗，持续8周。ZnO-NPs缓解了TAA诱导的肝脏或肾脏中还原型谷胱甘肽（GSH）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的减少。此外，ZnO-NPs降低了组织丙二醛（MDA，脂质过氧化指标）。TAA治疗导致血浆炎症标志物（TNF-α、IL-6）、肝酶（γ-谷氨酰转移酶（GGT）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT））以及肾功能参数（肌酐、尿素、尿酸）显著升高。然而，用ZnO-NPs治疗后这些参数降低。此外，ZnO-NPs降低了肝纤维化标志物、羟脯氨酸水平以及α-平滑肌肌动蛋白免疫阳性染色。肝和肾切片的组织病理学和免疫组织化学研究也证实了ZnO-NPs在生化变化方面的保护作用。我们的结果表明，ZnO-NPs可能通过抑制氧化应激来减轻TAA毒性。
