Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al- Ahsa, Saudi Arabia.
Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
BMC Vet Res. 2020 Sep 23;16(1):349. doi: 10.1186/s12917-020-02482-5.
The use of zinc oxide in the form of nanoparticles (ZnO-NPs) is of great benefit due to its potent effectiveness and higher bioavailability compared to zinc oxide. This study aimed to investigate the impact of dietary inclusion of different doses of ZnO-NPs on selected serum biomarkers, lipid peroxidation and tissue gene expression of antioxidant enzymes and cytokines in Japanese quail. Eighty Japanese quails (Coturnix japonica) (45 days old) were randomly divided into four groups (20 birds for each) with 4 replicates (5 birds each). Birds in the first group were fed a basal diet alone and served as a control (C). Birds in groups 2-4 were fed the basal diet supplemented with ZnO-NPs at doses of 15 mg/kg, 30 mg/kg and 60 mg/kg for a period of 60 days. At the end of the experiment, all birds were sacrificed to collect blood in a plain vacutainer, whereas liver and brain tissues were stored frozen at -80 °C. The obtained sera were used for the analysis of selected biochemical parameters, whereas tissue homogenates were used for the estimation of zinc, oxidative stress biomarkers and gene expression of selected antioxidant enzymes and cytokines.
ZnO-NPs (30 and 60 mg/kg) induced a significant decrease in serum triacylglycerol (TAG) compared to the control. ZnO-NPs did not affect the activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein, albumin, globulin and tissue zinc concentrations but reduced the malondialdehyde (MDA) levels compared to the control. The liver retained a higher zinc concentration than that of brain tissue. In a dose-dependent manner, ZnO-NPs upregulated the mRNA levels of antioxidant enzymes (superoxide dismutase: SOD1; catalase: CAT; glutathione peroxidase-1: GPX 1) and pro-inflammatory cytokines (interferon α: IFN-α; interleukin 6: IL-6) in liver and brain tissues.
The current study suggests the inclusion of ZnO-NPs, particularly 60 mg/kg, in the diet of Japanese quails to improve antioxidant and immune status.
氧化锌纳米粒子（ZnO-NPs）的使用具有很大的益处，因为与氧化锌相比，它具有更强的功效和更高的生物利用度。本研究旨在研究日粮中添加不同剂量的 ZnO-NPs 对鹌鹑某些血清生物标志物、脂质过氧化和组织抗氧化酶和细胞因子基因表达的影响。将 80 只日本鹌鹑（Coturnix japonica）（45 日龄）随机分为四组（每组 20 只，4 个重复，每个重复 5 只）。第一组的鸟类仅喂食基础日粮，作为对照（C）。第二组至第四组的鸟类在基础日粮中添加 15mg/kg、30mg/kg 和 60mg/kg 的 ZnO-NPs，为期 60 天。实验结束时，所有鸟类被处死，从普通真空管中采集血液，而肝脏和脑组织则储存在-80°C 的冷冻状态。所得血清用于分析选定的生化参数，而组织匀浆用于估计锌、氧化应激生物标志物和选定抗氧化酶和细胞因子的基因表达。
与对照组相比，ZnO-NPs（30 和 60mg/kg）显著降低了血清三酰甘油（TAG）。ZnO-NPs 对血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总蛋白、白蛋白、球蛋白和组织锌浓度的活性没有影响，但与对照组相比，降低了丙二醛（MDA）水平。肝脏比脑组织保留更高的锌浓度。以剂量依赖的方式，ZnO-NPs 上调了肝脏和脑组织中抗氧化酶（超氧化物歧化酶：SOD1；过氧化氢酶：CAT；谷胱甘肽过氧化物酶-1：GPX 1）和促炎细胞因子（干扰素-α：IFN-α；白细胞介素 6：IL-6）的 mRNA 水平。
本研究表明，在鹌鹑日粮中添加 ZnO-NPs，特别是 60mg/kg，可以改善抗氧化和免疫状态。
