Shinyama Shin, Kaji Tatsuru, Mukai Motoi, Nakame Kazuhiko, Matsufuji Hiroshi, Takamatsu Hideo, Ieiri Satoshi
Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, 8-35-1, Sakuragaoka, Kagoshima, 890-8520, Japan.
Ambulatory Care Center for Children, St. Luke's International Hospital, Tokyo, Japan.
Pediatr Surg Int. 2017 Oct;33(10):1109-1114. doi: 10.1007/s00383-017-4145-9. Epub 2017 Aug 16.
Necrotizing enterocolitis (NEC) is a devastating gastrointestinal disease of premature infants. Daikenchuto, a Japanese herbal drug, has several effects on the digestive system, so we investigated its preventive effects in a rat model of NEC.
NEC was induced in newborn rats via asphyxia (100% N for 90 s; every 4 h) + LPS (4 mg/kg/day [administered orally on days 0 and 1]). The effects of Daikenchuto were evaluated in four groups (control: 0 g/kg/day, I: 0.3 g/kg/day, II: 0.6 g/kg/day, and III: 1.0 g/kg/day). Daikenchuto was administered into the stomach through a microcatheter. The incidence and severity of NEC were pathologically assessed using the NEC grade in accordance with Dovorak's previous report. Cell positivity for inflammatory cytokine (IL-6) was also evaluated.
Daikenchuto reduced the incidence of NEC in control, Groups I, II, and III to 68.7, 30.0, 30.7, and 13.3%, respectively. High-dose Daikenchuto significantly improved the incidence of NEC, and the rate of IL-6 positive cells in group III was significantly lower than in the control group (p = 0.04).
We evaluated the effect of Daikenchuto against NEC and found that it reduced the incidence rate of NEC due to a decrease in the IL-6 production.
坏死性小肠结肠炎（NEC）是一种严重影响早产儿的胃肠道疾病。日本草药大建中汤对消化系统有多种作用，因此我们在NEC大鼠模型中研究了其预防作用。
通过窒息（100%氮气，90秒；每4小时一次）+脂多糖（4毫克/千克/天[在第0天和第1天口服给药]）诱导新生大鼠患NEC。在四组中评估大建中汤的作用（对照组：0克/千克/天，I组：0.3克/千克/天，II组：0.6克/千克/天，III组：1.0克/千克/天）。通过微导管将大建中汤注入胃中。根据多夫拉克之前的报告，使用NEC分级对NEC的发病率和严重程度进行病理评估。还评估了炎性细胞因子（IL-6）的细胞阳性率。
大建中汤将对照组、I组、II组和III组的NEC发病率分别降至68.7%、30.0%、30.7%和13.3%。高剂量大建中汤显著提高了NEC的发病率，III组中IL-6阳性细胞的比例显著低于对照组（p = 0.04）。
我们评估了大建中汤对NEC的作用，发现它由于IL-6产生的减少而降低了NEC的发病率。
