脂肪细胞分化中的自噬

Autophagy in Adipocyte Differentiation.

作者信息

Tao Zhipeng, Liu Longhua, Zheng Louise D, Cheng Zhiyong

机构信息

Department of Human Nutrition, Foods, and Exercise, Fralin Translational Obesity Research Center, College of Agriculture and Life Science, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA, 24061, USA.

Department of Pathology and Cell Biology, Naomi Berrie Diabetes Center, College of Physicians and Surgeons, Columbia University, Virginia Tech, 1981 Kraft Drive, New York, NY 10032, USA.

出版信息

Methods Mol Biol. 2019;1854:45-53. doi: 10.1007/7651_2017_65.

DOI:10.1007/7651_2017_65

PMID:28815517

Abstract

Adipose tissue regulates metabolic homeostasis by acting as an endocrine organ and energy reservoir. Adipose tissue development and functional maintenance are dependent on adipocyte differentiation, in which autophagy plays an important role. It has been shown that autophagy deficiency dampens adipocyte differentiation, compromises adipose tissue development, dysregulates adipocytokine secretion, and even causes sudden death in young animals. Therefore, accurate assessment of autophagy in adipocyte is critical for the study of adipose biology or pathology of metabolic diseases. In this chapter, we described the procedure of autophagy analysis during adipocyte differentiation, and discussed the power of steady-state autophagy protein (e.g., beclin 1, LC3, and p62) levels versus autophagy flux to reflect autophagy activity.

摘要

脂肪组织通过作为内分泌器官和能量储存库来调节代谢稳态。脂肪组织的发育和功能维持依赖于脂肪细胞分化，其中自噬起着重要作用。研究表明，自噬缺陷会抑制脂肪细胞分化、损害脂肪组织发育、失调脂肪细胞因子分泌，甚至导致幼龄动物猝死。因此，准确评估脂肪细胞中的自噬对于研究脂肪生物学或代谢疾病病理学至关重要。在本章中，我们描述了脂肪细胞分化过程中自噬分析的步骤，并讨论了稳态自噬蛋白（如贝林1、微管相关蛋白轻链3和p62）水平与自噬通量反映自噬活性的能力。