Tarongoy Faustino M, Haddad Paul R, Quirino Joselito P
Australian Centre for Research on Separation Science, School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Tasmania, Australia.
Chemistry Department, College of Arts and Sciences, Xavier University-Ateneo de Cagayan, Cagayan de Oro, Misamis Oriental, Philippines.
Electrophoresis. 2018 Jan;39(1):34-52. doi: 10.1002/elps.201700280. Epub 2017 Sep 6.
Interest in open-tubular capillary electrochromatography (OT-CEC) continues to thrive because of the inherent advantage of OT-CEC combining the high efficiency of capillary electrophoresis and the high selectivity of high performance liquid chromatography. For the period 2016 to 2017, novel materials have been developed as first-time stationary phases for OT-CEC and are grouped in this review as polymer-based materials, frameworks, nanoparticles, graphene-based materials, and biomaterials. Coating and fabrication methods mostly rely on covalent coating strategies while non-covalent immobilisation strategies like electrostatic assembly are notably still being employed. The concern of overcoming phase ratio challenges in OT-CEC coatings have also generated adoption of combined coating strategies including multi-layering, layer-by-layer self-assembly and methods adapted from nanofilm fabrications like epitaxial growth, liquid phase deposition, or nucleation of crystal growth. The emergence of non-conventional coating characterisation methods such as transmission electron microscopy, X-ray diffraction or X-ray photoelectron spectroscopy is also discussed.
由于开管毛细管电色谱（OT-CEC）结合了毛细管电泳的高效性和高效液相色谱的高选择性这一固有优势，人们对它的兴趣持续高涨。在2016年至2017年期间，新型材料被开发出来作为OT-CEC的首次固定相，并在本综述中分为基于聚合物的材料、框架材料、纳米颗粒、石墨烯基材料和生物材料。涂层和制备方法大多依赖于共价涂层策略，而静电组装等非共价固定策略仍在显著使用。克服OT-CEC涂层中相比挑战的问题也促使人们采用包括多层、逐层自组装以及从纳米薄膜制备方法改编而来的方法，如外延生长、液相沉积或晶体生长成核等组合涂层策略。本文还讨论了诸如透射电子显微镜、X射线衍射或X射线光电子能谱等非常规涂层表征方法的出现。
