评估不同声压水平对大鼠血清醛固酮浓度水平的影响。
Evaluation of the effects of various sound pressure levels on the level of serum aldosterone concentration in rats.
作者信息
Nassiri Parvin, Zare Sajad, Monazzam Mohammad R, Pourbakht Akram, Azam Kamal, Golmohammadi Taghi
机构信息
Department of Occupational Health, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Occupational Health, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran.
出版信息
Noise Health. 2017 Jul-Aug;19(89):200-206. doi: 10.4103/nah.NAH_64_16.
INTRODUCTION
Noise exposure may have anatomical, nonauditory, and auditory influences. Considering nonauditory impacts, noise exposure can cause alterations in the automatic nervous system, including increased pulse rates, heightened blood pressure, and abnormal secretion of hormones. The present study aimed at examining the effect of various sound pressure levels (SPLs) on the serum aldosterone level among rats.
MATERIALS AND METHODS
A total of 45 adult male rats with an age range of 3 to 4 months and a weight of 200 ± 50 g were randomly divided into 15 groups of three. Three groups were considered as the control groups and the rest (i.e., 12 groups) as the case groups. Rats of the case groups were exposed to SPLs of 85, 95, and 105 dBA. White noise was used as the noise to which the rats were exposed. To measure the level of rats' serum aldosterone, 3 mL of each rat's sample blood was directly taken from the heart of anesthetized animals by using syringes. The taken blood samples were put in labeled test tubes that contained anticoagulant Ethylenediaminetetraacetic acid. In the laboratory, the level of aldosterone was assessed through Enzyme-linked immunosorbent assay protocol. The collected data were analyzed by the use of Statistical Package for Social Sciences (SPSS) version 18.
RESULTS
The results revealed that there was no significant change in the level of rats' serum aldosterone as a result of exposure to SPLs of 65, 85, and 95 dBA. However, the level of serum aldosterone experienced a remarkable increase after exposure to the SPL of 105 dBA (P < 0.001). Thus, the SPL had a significant impact on the serum aldosterone level (P < 0.001). In contrast, the exposure time and the level of potassium in the used water did not have any measurable influence on the level of serum aldosterone (P = 0.25 and 0.39).
CONCLUSION
The findings of this study demonstrated that serum aldosterone can be used as a biomarker in the face of sound exposure.
引言
噪声暴露可能会对解剖结构、非听觉系统和听觉系统产生影响。考虑到非听觉方面的影响，噪声暴露会导致自主神经系统发生变化，包括脉搏加快、血压升高以及激素分泌异常。本研究旨在探讨不同声压级（SPL）对大鼠血清醛固酮水平的影响。
材料与方法
总共45只3至4个月大、体重200±50克的成年雄性大鼠被随机分成15组，每组3只。其中3组被视为对照组，其余12组为实验组。实验组的大鼠分别暴露于85、95和105分贝的声压级环境中。使用白噪声作为大鼠暴露的噪声。为测量大鼠血清醛固酮水平，通过注射器直接从麻醉动物的心脏采集每只大鼠3毫升的血样。采集的血样放入含有抗凝剂乙二胺四乙酸的标记试管中。在实验室中，通过酶联免疫吸附测定法评估醛固酮水平。收集的数据使用社会科学统计软件包（SPSS）18版进行分析。
结果
结果显示，暴露于65、85和95分贝的声压级环境中，大鼠血清醛固酮水平无显著变化。然而，暴露于105分贝的声压级环境后，血清醛固酮水平显著升高（P < 0.001）。因此，声压级对血清醛固酮水平有显著影响（P < 0.001）。相比之下，暴露时间和所用水中的钾含量对血清醛固酮水平没有任何可测量的影响（P = 0.25和0.39）。
结论
本研究结果表明，血清醛固酮可作为噪声暴露情况下的生物标志物。
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