Department of Biomedical Sciences, Catholic Kwandong University, Gangneung, Republic of Korea.
Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
J Cell Physiol. 2018 Apr;233(4):3129-3140. doi: 10.1002/jcp.26150. Epub 2017 Nov 14.
Chrysin is mainly found in passion flowers, honey, and propolis acts as a potential therapeutic and preventive agent to inhibit proliferation and invasion of various human cancer cells. Although chrysin has anti-carcinogenic effects in several cancers, little is known about its functional roles in ovarian cancer which shows poor prognosis and chemoresistance to traditional therapeutic agents. In the present study, we investigated functional roles of chrysin in progression of ovarian cancer cells using ES2 and OV90 (clear cell and serous carcinoma, respectively) cell lines. Results of the current study demonstrated that chrysin inhibited ovarian cancer cell proliferation and induced cell death by increasing reactive oxygen species (ROS) production and cytoplasmic Ca levels as well as inducing loss of mitochondrial membrane potential (MMP). Moreover, chrysin activated mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/AKT pathways in ES2 and OV90 cells in concentration-response experiments. Collectively, our results led us to propose that chrysin-induced apoptotic events are mediated by the activation of PI3K and MAPK pathways in human ovarian cancer cells.
白杨素主要存在于西番莲、蜂蜜和蜂胶中，作为一种有潜力的治疗和预防剂，可抑制多种人类癌细胞的增殖和侵袭。尽管白杨素有几种癌症的抗癌作用，但对其在卵巢癌中的功能作用知之甚少，卵巢癌预后不良，对传统治疗药物有耐药性。在本研究中，我们使用 ES2 和 OV90（分别为透明细胞癌和浆液性癌）细胞系研究了白杨素在卵巢癌细胞进展中的功能作用。本研究结果表明，白杨素通过增加活性氧（ROS）的产生和细胞质 Ca 水平以及诱导线粒体膜电位（MMP）丧失来抑制卵巢癌细胞增殖并诱导细胞死亡。此外，在 ES2 和 OV90 细胞的浓度反应实验中，白杨素激活了丝裂原活化蛋白激酶（MAPK）和磷酸肌醇 3-激酶（PI3K）/AKT 通路。综上所述，我们的研究结果表明，白杨素诱导的凋亡事件是通过人卵巢癌细胞中 PI3K 和 MAPK 通路的激活介导的。
