Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Department of Biomedical Sciences, Catholic Kwandong University, Gangneung, Republic of Korea.
J Cell Physiol. 2018 Nov;233(11):8597-8604. doi: 10.1002/jcp.26540. Epub 2018 Jun 15.
Sideroxylin is a C-methylated flavone isolated from Callistemon lanceolatus and exerts antimicrobial activity against Staphylococcus aureus. However, the anticancer effects of sideroxylin and its intracellular signaling mechanisms have not yet been identified. Results of our study showed that sideroxylin decreased cell proliferation and increased apoptosis, causing DNA fragmentation, depolarization of the mitochondrial membrane, the generation of reactive oxygen species, and an increase of lipid peroxidation in ovarian cancer cells (ES2 and OV90 cells). Additionally, sideroxylin activated the phosphorylation of ERK1/2, JNK, P38, and MAPK proteins and the use of LY294002, U0126, SB203580, and SP600125 to block their phosphorylation, respectively, in ES2 and OV90 cells. Collectively, the results of present study indicated that sideroxylin was a novel therapeutic agent to combat the proliferation of ovarian cancer cells through the induction of mitochondrial dysfunction and the activation of PI3 K and MAPK signal transduction.
紫檀芪是从蓝花楹中分离得到的 C-甲基化黄酮，具有抗金黄色葡萄球菌的活性。然而，紫檀芪的抗癌作用及其细胞内信号机制尚未得到确定。我们的研究结果表明，紫檀芪可降低细胞增殖并诱导细胞凋亡，导致 DNA 片段化、线粒体膜去极化、活性氧生成和脂质过氧化增加，从而对卵巢癌细胞（ES2 和 OV90 细胞）产生作用。此外，紫檀芪可激活 ERK1/2、JNK、P38 和 MAPK 蛋白的磷酸化，使用 LY294002、U0126、SB203580 和 SP600125 分别阻断 ES2 和 OV90 细胞中它们的磷酸化。总之，本研究结果表明，紫檀芪通过诱导线粒体功能障碍和激活 PI3K 和 MAPK 信号转导，是一种新型的治疗卵巢癌细胞增殖的药物。
