Mukhtar M Shahid, Liu Xiaoyu, Somssich Imre E
a Department of Biology , University of Alabama at Birmingham , AL , USA.
b Nutrition Obesity Research Center , University of Alabama at Birmingham , AL , USA.
Plant Signal Behav. 2017 Sep 2;12(9):e1363945. doi: 10.1080/15592324.2017.1363945. Epub 2017 Aug 17.
The WRKY proteins belong to a superfamily of TFs that play pivotal roles in responses to a wide range of biotic, abiotic, developmental and physiologic cues. Here, we assayed the accumulation of basal WRKY27 transcripts in diverse tissue including root, shoot, leaf and flowers. We demonstrated that plants over-expressing WRKY27 transcript levels exhibit growth aberrations and fertility defects. Scanning electron microscopic data suggest that WRKY27 overexpressor plants exhibit pollen dehiscence defects. Our fluorescein diacetate hydrolysis assay showed that flowers of plants overexpressing WRKY27 display significantly decreased pollen viability. These sterility-related phenotypes were not rescued by the exogenous applications of different phytohormones. Our results indicate the involvement of WRKY27 in particular for proper plant biomass accumulation and male fertility.
WRKY蛋白属于转录因子超家族，在对多种生物、非生物、发育和生理信号的响应中起关键作用。在此，我们检测了基础WRKY27转录本在包括根、茎、叶和花在内的不同组织中的积累情况。我们证明，过表达WRKY27转录水平的植物表现出生长异常和育性缺陷。扫描电子显微镜数据表明，WRKY27过表达植物表现出花粉开裂缺陷。我们的荧光素二乙酸酯水解试验表明，过表达WRKY27的植物花朵显示出花粉活力显著下降。不同植物激素的外源施用未能挽救这些与不育相关的表型。我们的结果表明WRKY27特别参与了植物生物量的正常积累和雄性育性。
