Cryer J, Woodhead B G, Cryer A
Comp Biochem Physiol A Comp Physiol. 1987;86(3):515-21. doi: 10.1016/0300-9629(87)90535-4.
The conditions of isolation and culture of a chicken adipose stromal-derived cell strain are described and compared with chicken lung fibroblasts in vitro. The stromal cells accumulated intracellular lipid during the post-confluency culture period, this being by contrast with lung fibroblasts. Much higher levels of intracellular lipid were accumulated by the stromal cells when whole chicken serum or chicken plasma lipoproteins were added to the culture medium. Lipoprotein lipase activity emerged in stromal cells maintained post confluency. This activity was absent from pre-confluent stromal cells and pre- and post-confluent fibroblasts. The incorporation of 14C-acetate, 3H-oleic acid and 14C-glucose into lipids by the stromal cells exhibited a pattern compatible with a concerted shift in the metabolism of the cells towards lipid storage, particularly in the form of triacylglycerols derived from exogenous fatty acids. It is proposed that, in common with the mammalian species previously studied, the white adipose tissue of the chicken (Gallus domesticus) contains a cell type with properties which allow its preliminary identification as an adipocyte precursor cell capable of adipose conversion in vitro. The confirmation of this proposition is amenable to further investigation.
描述了鸡脂肪基质衍生细胞系的分离和培养条件，并在体外与鸡肺成纤维细胞进行了比较。在汇合后培养期，基质细胞在细胞内积累脂质，这与肺成纤维细胞形成对比。当向培养基中添加全鸡血清或鸡血浆脂蛋白时，基质细胞积累的细胞内脂质水平更高。脂蛋白脂肪酶活性在汇合后培养的基质细胞中出现。在汇合前的基质细胞以及汇合前和汇合后的成纤维细胞中均未发现这种活性。基质细胞将14C-乙酸盐、3H-油酸和14C-葡萄糖掺入脂质的模式表明，细胞代谢朝着脂质储存发生了协同转变，特别是以源自外源性脂肪酸的三酰甘油形式。有人提出，与先前研究的哺乳动物物种一样，鸡（家鸡）的白色脂肪组织含有一种细胞类型，其特性使其初步鉴定为能够在体外进行脂肪转化的脂肪细胞前体细胞。这一命题的证实有待进一步研究。
