García Antonio A, Franco Lina S, Pirez-Gomez Miguel A, Pech-Pacheco José L, Mendez-Galvan Jorge F, Machain-Williams Carlos, Talavera-Aguilar Lourdes, Espinosa-Carrillo José H, Duarte-Villaseñor Miriam M, Be-Ortiz Christian, Espinosa-de Los Monteros Luz E, Castillo-Pacheco Ariel, Garcina-Rejon Julian E
School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA.
School of Molecular Sciences, Arizona State University, Tempe, AZ 85287, USA.
Diagnostics (Basel). 2017 Aug 17;7(3):47. doi: 10.3390/diagnostics7030047.
Antibody detection and accurate diagnosis of tropical diseases is essential to help prevent the spread of disease. However, most detection methods lack cost-effectiveness and field portability, which are essential features for achieving diagnosis in a timely manner. To address this, 3D-printed oblate spheroid sample chambers were fabricated to measure green light scattering of gold nanoparticles using an optical caustic focus to detect antibodies. Scattering signals of 20-200 nm gold nanoparticles using a green laser were compared to green light emitting diode (LED) light source signals and to Mie theory. The change in signal from 60 to 120 nm decreased in the order of Mie Theory > optical caustic scattering > 90° scattering. These results suggested that conjugating 60 nm gold nanoparticles and using an optical caustic system to detect plasmonic light scattering, would result in a sensitive test for detecting human antibodies in serum. Therefore, we studied the light scattering response of conjugated gold nanoparticles exposed to different concentrations of anti-protein E antibody, and a feasibility study of 10 human serum samples using dot blot and a handheld optical caustic-based sensor device. The overall agreement between detection methods suggests that the new sensor concept shows promise to detect gold nanoparticle aggregation in a homogeneous assay. Further testing and protocol optimization is needed to draw conclusions on the positive and negative predictive values for this new testing system.
热带疾病的抗体检测和准确诊断对于预防疾病传播至关重要。然而，大多数检测方法缺乏成本效益和现场便携性，而这些是及时进行诊断的关键特性。为了解决这一问题，制造了3D打印的扁球体样品室，利用光学焦散聚焦来测量金纳米颗粒的绿光散射以检测抗体。将使用绿色激光的20 - 200 nm金纳米颗粒的散射信号与绿色发光二极管（LED）光源信号以及米氏理论进行了比较。从60到120 nm信号的变化按米氏理论>光学焦散散射>90°散射的顺序递减。这些结果表明，结合60 nm金纳米颗粒并使用光学焦散系统来检测等离子体光散射，将产生一种用于检测血清中人类抗体的灵敏测试方法。因此，我们研究了结合的金纳米颗粒在不同浓度抗蛋白E抗体作用下的光散射响应，并使用斑点印迹法和基于手持式光学焦散的传感器装置对10份人类血清样本进行了可行性研究。检测方法之间的总体一致性表明，这种新的传感器概念有望在均相分析中检测金纳米颗粒的聚集。需要进一步测试和优化方案，以便对这种新测试系统的阳性和阴性预测值得出结论。
