Thorn Robert J, Clift Danielle E, Ojo Oladele, Colwill Ruth M, Creton Robbert
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, United States of America.
Department of Cognitive, Linguistic and Psychological Sciences, Brown University, Providence, Rhode Island, United States of America.
PLoS One. 2017 Aug 17;12(8):e0183414. doi: 10.1371/journal.pone.0183414. eCollection 2017.
Regenerative medicine offers potentially ground-breaking treatments of blindness and low vision. However, as new methodologies are developed, a critical question will need to be addressed: how do we monitor in vivo for functional success? In the present study, we developed novel behavioral assays to examine vision in a vertebrate model system. In the assays, zebrafish larvae are imaged in multiwell or multilane plates while various red, green, blue, yellow or cyan objects are presented to the larvae on a computer screen. The assays were used to examine a loss of vision at 4 or 5 days post-fertilization and a gradual recovery of vision in subsequent days. The developed assays are the first to measure the loss and recovery of vertebrate vision in microplates and provide an efficient platform to evaluate novel treatments of visual impairment.
再生医学为失明和视力低下提供了具有潜在突破性的治疗方法。然而，随着新方法的不断发展，一个关键问题亟待解决：我们如何在体内监测功能是否成功？在本研究中，我们开发了新的行为分析方法来检测脊椎动物模型系统中的视力。在这些分析中，斑马鱼幼体在多孔板或多通道板中成像，同时在电脑屏幕上向幼体呈现各种红色、绿色、蓝色、黄色或青色的物体。这些分析方法用于检测受精后4或5天的视力丧失情况以及随后几天视力的逐渐恢复情况。所开发的分析方法是首次在微孔板中测量脊椎动物视力的丧失和恢复情况，并为评估视觉障碍的新治疗方法提供了一个有效的平台。
