Zhang Dayong, Huang Yihong, Kumar Manoj, Wan Qun, Xu Zhaolong, Shao Hongbo, Pandey Girdhar K
Salt-Soil Agricultural Center, Institute of Agricultural Resources and Environment Jiangsu Academy of Agricultural Sciences, Zhongling Street No.50, Nanjing 210014, China.
Salt-Soil Agricultural Center, Institute of Agricultural Resources and Environment Jiangsu Academy of Agricultural Sciences, Zhongling Street No.50, Nanjing 210014, China.
Plant Sci. 2017 Oct;263:210-218. doi: 10.1016/j.plantsci.2017.07.018. Epub 2017 Jul 27.
Aquaporins (AQPs) are transmembrane protein channels that are members of Major Intrinsic Proteins (MIP) superfamily. AQPs play important roles in plant reproduction, cell elongation, osmoregulation, influence leaf physiology and are responsive to drought and salt tolerance. Small intrinsic proteins (SIPs)belongs to one of the groups of AQPs, which are mainly localized to endoplasmic reticulum(ER). While this group of aquaporin is being well studied in Arabidopsis, grape and other plant species, not much is known about the molecular regulatory mechanisms driven by ER-type AQPs in Glycine Max. In this study, the function of GmSIP1;3 is studied in detail by using both yeast and plant systems. GmSIP1;3 showed a ubiquitous expression pattern in all different tissues in Glycine Max. Heterologous expression of GmSIP1;3 in Nicotiana tabacum conferred a short root phenotype,growth retardation at seedling stage and significant tolerance to oxidative stress (HO) both in yeast and plant systems. Auxin (IAA) content significantly increased in transgenic plants compared with that of wild type, however, the abscisic acid (ABA) content was significantly reduced. Subcellular localization and colocalization analyses showed GmSIP1;3 localized to ER plasma membrane. On the basis of these observations, we postulate that GmSIP1;3 is involved in oxidative stress pathways.
水通道蛋白（AQPs）是跨膜蛋白通道，属于主要内在蛋白（MIP）超家族成员。水通道蛋白在植物繁殖、细胞伸长、渗透调节、影响叶片生理以及对干旱和耐盐性方面发挥着重要作用。小内在蛋白（SIPs）属于水通道蛋白家族之一，主要定位于内质网（ER）。虽然这一类水通道蛋白在拟南芥、葡萄和其他植物物种中已得到充分研究，但关于内质网型水通道蛋白在大豆中驱动的分子调控机制却知之甚少。在本研究中，通过酵母和植物系统详细研究了GmSIP1;3的功能。GmSIP1;3在大豆的所有不同组织中均呈现普遍表达模式。GmSIP1;3在烟草中的异源表达导致了短根表型、幼苗期生长迟缓以及在酵母和植物系统中对氧化应激（HO）具有显著耐受性。与野生型相比，转基因植物中的生长素（IAA）含量显著增加，然而，脱落酸（ABA）含量显著降低。亚细胞定位和共定位分析表明，GmSIP1;3定位于内质网 - 质膜。基于这些观察结果，我们推测GmSIP1;3参与氧化应激途径。
