Miyachi Y, Yoshioka A, Imamura S, Niwa Y
Gut. 1987 Feb;28(2):190-5. doi: 10.1136/gut.28.2.190.
The relative in vitro anti-oxidant efficacy of sulphasalazine (salicylazosulphapyridine, SASP) and its metabolites (5-aminosalicylic acid, 5-ASA; sulphapyridine, SP) was examined by studying their effects on the generation of reactive oxygen species (ROS) using zymosan-stimulated polymorphonuclear leucocytes (PMNs) and a cell free, xanthine-xanthine oxidase system. Salicylazosulphapyridine, 5-ASA, and SP showed anti-oxidant effects to the various degrees. In particular, production of OH, which is one of the most potent reactive oxygen species, was remarkably suppressed by 5-ASA dose relatedly. These findings suggest that SASP and its metabolites play an important role in the inhibition of respiratory bursts. As the potent products of the respiratory burst by polymorphonuclear leucocytes are thought to be important inflammatory mediators, suppression of toxic reactive oxygen species generation by these agents may partly explain the therapeutic efficacy of SASP in ulcerative colitis, which is characterised by an acute mucosal inflammation dominated by polymorphonuclear leucocytes accumulation.
通过研究柳氮磺胺吡啶（水杨酸偶氮磺胺吡啶，SASP）及其代谢产物（5-氨基水杨酸，5-ASA；磺胺吡啶，SP）对酵母聚糖刺激的多形核白细胞（PMN）产生活性氧（ROS）的影响以及对无细胞的黄嘌呤-黄嘌呤氧化酶系统的影响，来检测它们在体外的相对抗氧化功效。水杨酸偶氮磺胺吡啶、5-ASA和SP均表现出不同程度的抗氧化作用。特别是，5-ASA能剂量依赖性地显著抑制最具活性的活性氧之一——羟基自由基（OH）的产生。这些发现表明，SASP及其代谢产物在抑制呼吸爆发中起重要作用。由于多形核白细胞呼吸爆发的强效产物被认为是重要的炎症介质，这些药物对毒性活性氧产生的抑制作用可能部分解释了SASP在溃疡性结肠炎中的治疗效果，溃疡性结肠炎的特征是急性黏膜炎症，以多形核白细胞积聚为主。
