Miyachi Y, Yoshioka A, Imamura S, Niwa Y
Br J Dermatol. 1987 Jan;116(1):39-46. doi: 10.1111/j.1365-2133.1987.tb05789.x.
The anti-oxidant efficacy, in vitro, of the gold compounds auranofin (AF) and gold sodium thiomalate (GST) was examined by studying their effects on the generation of reactive oxygen species (ROS) using zymosan-stimulated polymorphonuclear leukocytes (PMNs) and a cell-free, xanthine-xanthine oxidase system. The oxygen species investigated were the superoxide radical anion (O2-), hydrogen peroxide (H2O2) and the hydroxyl radical (OH.). AF had an inhibitory effect on ROS production by PMNs. In particular, OH. generation was significantly suppressed in a dose-dependent fashion. AF did not inhibit ROS production in the cell-free system. GST produced only a small degree of inhibition at higher concentrations. These findings suggest that AF may play an important role in the inhibition of respiratory bursts and the generation of inflammatory reaction products. Since the products of the respiratory burst, especially potent oxidants such as OH. and H2O2, are thought to be important inflammatory mediators, it is postulated that the blockade of toxic ROS generation by AF affects rheumatoid as well as dermatological inflammation and tissue damage.
通过研究金化合物金诺芬（AF）和硫代苹果酸金钠（GST）对酵母聚糖刺激的多形核白细胞（PMN）产生活性氧（ROS）的影响以及对无细胞黄嘌呤-黄嘌呤氧化酶系统的影响，在体外检测了它们的抗氧化功效。所研究的氧种类包括超氧阴离子自由基（O2-）、过氧化氢（H2O2）和羟基自由基（OH·）。AF对PMN产生ROS有抑制作用。特别是，OH·的产生以剂量依赖方式被显著抑制。AF在无细胞系统中不抑制ROS产生。GST在较高浓度时仅产生轻微抑制作用。这些发现表明AF可能在抑制呼吸爆发和炎症反应产物生成中起重要作用。由于呼吸爆发的产物，尤其是如OH·和H2O2等强效氧化剂，被认为是重要的炎症介质，因此推测AF对有毒ROS生成的阻断会影响类风湿性炎症以及皮肤炎症和组织损伤。
