Gionchetti P, Guarnieri C, Campieri M, Belluzzi A, Brignola C, Iannone P, Miglioli M, Barbara L
Dipartimento di Biochimica, Università di Bologna, Italy.
Dig Dis Sci. 1991 Feb;36(2):174-8. doi: 10.1007/BF01300752.
The in vitro antioxidant capacity of sulfasalazine (SASP), its metabolites (SP, 5-ASA), and olsalazine (OAZ), was studied by evaluating their effects on superoxide (O2-.) production. Assay systems were the xanthine-xanthine oxidase (X/XOD) reaction and phorbol myristate acetate (PMA)-activated polymorphonuclear leukocytes (PMNs), using the cytochrome c (cyt-c) reduction assay and a luminol-dependent chemiluminescence method. 5-ASA, SASP, and OAZ showed a dose-dependent scavenger effect in both O2-. generating systems, 5-ASA being the most powerful (greater than 50% of inhibition in the PMNs system and greater than 70% in the X/XOD system at 10 microM concentration). SP had an inhibitory effect only in the PMNs system but did not modify the activity of xanthine oxidase, thus excluding a scavenger action. These data suggest that the scavenger effect of 5-ASA, SASP, and OAZ may be an important mechanism of action.
通过评估柳氮磺胺吡啶（SASP）、其代谢产物（SP、5-氨基水杨酸）和奥沙拉嗪（OAZ）对超氧阴离子（O2-.）产生的影响，研究了它们的体外抗氧化能力。检测系统采用黄嘌呤-黄嘌呤氧化酶（X/XOD）反应和佛波酯（PMA）激活的多形核白细胞（PMN），使用细胞色素c（cyt-c）还原检测法和鲁米诺依赖性化学发光法。5-氨基水杨酸、柳氮磺胺吡啶和奥沙拉嗪在两种O2-.生成系统中均表现出剂量依赖性清除作用，5-氨基水杨酸作用最强（在PMN系统中浓度为10 microM时抑制率大于50%，在X/XOD系统中大于70%）。SP仅在PMN系统中有抑制作用，但不改变黄嘌呤氧化酶的活性，因此排除了清除作用。这些数据表明，5-氨基水杨酸、柳氮磺胺吡啶和奥沙拉嗪的清除作用可能是一种重要的作用机制。
