MRC Integrative Epidemiology Unit, University of Bristol, Oakfield House, Oakfield Grove, Bristol BS8 2BN, UK; School of Social and Community Medicine, University of Bristol, Oakfield House, Oakfield Grove, Bristol BS8 2BN, UK.
School of Social and Community Medicine, University of Bristol, Oakfield House, Oakfield Grove, Bristol BS8 2BN, UK.
Mitochondrion. 2018 Mar;39:9-19. doi: 10.1016/j.mito.2017.08.007. Epub 2017 Aug 15.
The mitochondrial genome is present at variable copy number between individuals. Mitochondria are vulnerable to oxidative stress, and their dysfunction may be associated with cardiovascular disease. The association of mitochondrial DNA copy number with cardiometabolic risk factors (lipids, glycaemic traits, inflammatory markers, anthropometry and blood pressure) was assessed in two independent cohorts of European origin women, one in whom outcomes were measured at mean (SD) age 30 (4.3) years (N=2278) and the second at 69.4 (5.5) years (N=2872). Mitochondrial DNA copy number was assayed by quantitative polymerase chain reaction. Associations were adjusted for smoking, sociodemographic status, laboratory factors and white cell traits. Out of a total of 12 outcomes assessed in both cohorts, mitochondrial DNA copy number showed little or no association with the majority (point estimates were close to zero and nearly all p-values were >0.01). The strongest evidence was for an inverse association in the older cohort with insulin (standardised beta [95%CI]: -0.06, [-0.098, -0.022], p=0.002), but this association did not replicate in the younger cohort. Our findings do not provide support for variation in mitochondrial DNA copy number having an important impact on cardio-metabolic risk factors in European origin women.
线粒体基因组在个体之间的拷贝数存在差异。线粒体容易受到氧化应激的影响，其功能障碍可能与心血管疾病有关。在两个欧洲裔女性独立队列中，评估了线粒体 DNA 拷贝数与心血管代谢危险因素（脂质、血糖特征、炎症标志物、人体测量学和血压）之间的关联，其中一个队列的结局在平均（标准差）年龄 30（4.3）岁时进行测量（N=2278），第二个队列在 69.4（5.5）岁时进行测量（N=2872）。通过定量聚合酶链反应测定线粒体 DNA 拷贝数。调整了吸烟、社会人口统计学状况、实验室因素和白细胞特征。在两个队列共评估的 12 个结局中，线粒体 DNA 拷贝数与大多数结局几乎没有关联（点估计值接近零，几乎所有 p 值均大于 0.01）。在年龄较大的队列中，与胰岛素的关联最强（标准化β[95%CI]：-0.06，[-0.098，-0.022]，p=0.002），但这种关联在年轻队列中没有复制。我们的研究结果不支持线粒体 DNA 拷贝数的变异对欧洲裔女性的心血管代谢危险因素有重要影响。
